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PMID: 6325164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Herpes simplex virus infection causes the accumulation of a heat-shock protein.

The EMBO journal ·Vol. 3 ·No. 2 ·1984-02-00 ·Pages 267-77

LaThangue NB, Shriver K, Dawson C, Chan WL

Abstract

A monoclonal antibody, produced from mice immunized with a herpes simplex virus (HSV)-infected cell extract, reacts with a molecule which is present in uninfected cells and which accumulates in large amounts during HSV 2 infection. In uninfected cells this molecule is growth regulated, in that exponentially growing cells have intense nuclear immunofluorescence, whereas confluent quiescent cells have little. It has a mol. wt. of 57 000 (p57) in exponential cells, and one of 61 000 (p61) in quiescent cells. In HSV 2-infected cells, p57 accumulates and nuclear and cytoplasmic immunofluorescence increases. In uninfected cells, p57 also accumulates during heat-shock treatment, and this is associated with a new immunofluorescence throughout the cytoplasm. We suggest that HSV 2 infection induces a cellular stress response which is involved in the shut-off of host cell polypeptide synthesis.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Cell Line Cell Nucleus/analysis Cricetinae Embryo, Mammalian Fibroblasts/analysis,physiology Fluorescent Antibody Technique Heat-Shock Proteins/analysis Humans Kidney Mesocricetus Protein Biosynthesis Simplexvirus/physiology
Chemicals
Antibodies, Monoclonal Heat-Shock Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
LaThangue N B
Shriver K
Dawson C
Chan W L
References (37)
37 references, click to expand
  1. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  2. Degradation of cellular mRNA during infection by herpes simplex virus.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2370-4 PMID: 196289
  3. Monoclonal antibodies to herpes simplex viruses: use in antigenic typing and rapid diagnosis.
    J Infect Dis. 1983 May;147(5):829-37 PMID: 6302177
  4. T antigen is bound to a host protein in SV40-transformed cells.
    Nature. 1979 Mar 15;278(5701):261-3 PMID: 218111
  5. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  6. Intracellular localization of heat shock proteins in Drosophila.
    Cell. 1980 Jul;20(3):679-89 PMID: 6774811
  7. Enemies within: the genesis of retrovirus oncogenes.
    Cell. 1981 Jan;23(1):5-6 PMID: 7214527
  8. Herpes simplex virus types 1 and 2 induce shutoff of host protein synthesis by different mechanisms in Friend erythroleukemia cells.
    J Virol. 1983 Jan;45(1):241-50 PMID: 6296433
  9. Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1708-11 PMID: 6785759
  10. A comparison of the multiple Drosophila heat shock proteins in cell lines and larval salivary glands by two-dimensional gel electrophoresis.
    J Mol Biol. 1982 Jun 25;158(2):181-201 PMID: 6811757
  11. The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation.
    J Immunol Methods. 1976;12(1-2):171-81 PMID: 62811
  12. Polysomes and protein synthesis in cells infected with a DNA virus.
    Science. 1966 Jul 1;153(3731):76-8 PMID: 4287165
  13. Simian virus 40 and polyoma virus induce synthesis of heat shock proteins in permissive cells.
    Mol Cell Biol. 1983 Jan;3(1):1-8 PMID: 6298601
  14. Effect of antabuse (disulfiram) on Rous sarcoma virus and on eukaryotic cells.
    Biochim Biophys Acta. 1978 Jun 22;519(1):65-75 PMID: 78722
  15. Purification and biochemical characterization of the Epstein-Barr virus-determined nuclear antigen and an associated protein with a 53,000-dalton subunit.
    J Virol. 1980 Sep;35(3):592-602 PMID: 6158579
  16. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
    Cell. 1982 Feb;28(2):387-94 PMID: 6277513
  17. Heat-shock proteins are associated with hnRNA in Drosophila melanogaster tissue culture cells.
    EMBO J. 1983;2(5):705-10 PMID: 16453451
  18. Spontaneous high expression of heat-shock proteins in mouse embryonal carcinoma cells and ectoderm from day 8 mouse embryo.
    EMBO J. 1983;2(2):173-7 PMID: 11894922
  19. Newcastle disease virus stimulates the cellular accumulation of stress (heat shock) mRNAs and proteins.
    J Virol. 1982 Nov;44(2):703-7 PMID: 7143579
  20. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  21. Protective immunization of mice with specific HSV-1 glycoproteins.
    Immunology. 1983 Jun;49(2):343-52 PMID: 6303950
  22. The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts.
    Cell. 1978 Dec;15(4):1277-86 PMID: 569556
  23. Antigenic differences between isolates of herpesvirus type 2.
    Intervirology. 1974;3(1-2):1-14 PMID: 4474145
  24. The oncogenic potential of herpes simplex viruses: evidence for a 'hit-and-run' mechanism.
    Nature. 1983 Mar 3;302(5903):21-4 PMID: 6298634
  25. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
    Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
  26. Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene product.
    Cell. 1982 Jul;29(3):913-9 PMID: 7151177
  27. Is the major Drosophila heat shock protein present in cells that have not been heat shocked?
    J Cell Biol. 1983 Jan;96(1):286-90 PMID: 6826649
  28. Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.
    J Virol. 1974 Jul;14(1):8-19 PMID: 4365321
  29. Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species.
    Mol Cell Biol. 1982 Mar;2(3):267-74 PMID: 7110134
  30. Recovery of herpes simplex virus from human sacral ganglions.
    N Engl J Med. 1974 Oct 17;291(16):828-30 PMID: 4371760
  31. Activation of early adenovirus transcription by the herpesvirus immediate early gene: evidence for a common cellular control factor.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4952-6 PMID: 6289326
  32. Mouse hybrid cell lines produce antibodies to herpes simplex virus type 1.
    J Gen Virol. 1979 Jul;44(1):81-7 PMID: 228002
  33. Herpes simplex virus mutants defective in the virion-associated shutoff of host polypeptide synthesis and exhibiting abnormal synthesis of alpha (immediate early) viral polypeptides.
    J Virol. 1983 May;46(2):498-512 PMID: 6302315
  34. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  35. Suppression of the synthesis of cellular macromolecules by herpes simplex virus.
    J Gen Virol. 1978 Oct;41(1):37-51 PMID: 212520
  36. Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides.
    Virology. 1982 Nov;123(1):113-22 PMID: 6293187
  37. Two-dimensional gel analysis of HSV type 1-induced polypeptides and glycoprotein processing.
    J Gen Virol. 1981 Jan;52(Pt 1):77-92 PMID: 6267177
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-02-00
Pages
267-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557334
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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