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PMID: 6326141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An unusual 5' splice sequence is efficiently utilized in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 9 ·1984-05-00 ·Pages 2733-7

Fischer HD, Dodgson JB, Hughes S, Engel JD

Abstract

The minor adult chicken alpha-globin gene (alpha D) has an intron splicing sequence at the 5' end of the second intron that begins with the dinucleotide G-C rather than the usual G-T. To understand what role this splice sequence plays in the processing and maturation of nuclear RNA to cytoplasmic RNA, we have analyzed the intron processing of the alpha D-globin transcript in both heterologous (monkey) and homologous (chicken) cells using simian virus 40 and retrovirus vectors, respectively. In both cell types, both introns of the alpha D-globin gene are efficiently and precisely removed.

MeSH Terms
Animals Avian Leukosis Virus/genetics Base Sequence Chickens Chromosome Mapping Endonucleases Genetic Vectors Globins/genetics RNA Splicing Simian virus 40/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Globins Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fischer H D
Dodgson J B
Hughes S
Engel J D
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.9.2733
Published
1984-05-00
Pages
2733-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345144
Subset
IM
Grants
NCI NIH HHS · CA29569 · United States
NIGMS NIH HHS · GM28837 · United States
NHLBI NIH HHS · HL24415 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.9.2733 PMC Full Text View journal details
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