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PMID: 6327270 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of two distinct renin genes from the DBA/2 mouse.

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1461-6

Mullins JJ, Burt DW, Windass JD, McTurk P, George H, Brammar WJ

Abstract

We report the molecular cloning of cDNA copies of DBA/2 mouse submaxillary gland (SMG) renin mRNA, which were used to probe Southern transfers of mouse genomic DNA. The results suggested either that there is a single renin gene containing a large intron in that part of the gene corresponding to the probe, or that there are two distinct renin genes. We have shown that the latter is the case by cloning and isolating two similar but distinct renin genes from DBA/2 mouse DNA. Restriction maps of the regions containing the two renin genes are presented, together with nucleotide sequence data locating a complete exon coding for amino acids 268-315 of mouse SMG renin.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Isoenzymes/genetics Liver/enzymology Male Mice Mice, Inbred DBA Plasmids Renin/genetics Submandibular Gland/enzymology
Chemicals
Isoenzymes DNA DNA Restriction Enzymes Renin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mullins J J
Burt D W
Windass J D
McTurk P
George H
Brammar W J
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1461-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553232
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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