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PMID: 6327610 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of cell division in Escherichia coli: SOS induction and cellular location of the sulA protein, a key to lon-associated filamentation and death.

Journal of bacteriology ·Vol. 158 ·No. 2 ·1984-05-00 ·Pages 551-61

Schoemaker JM, Gayda RC, Markovitz A

Abstract

Mutations in sulA (sfiA) block the filamentation and death of capR (lon) mutants that occur after treatments that either damage DNA or inhibit DNA replication and thereby induce the SOS response. Previous sulA-lacZ gene fusion studies showed that sulA is transcriptionally regulated by the SOS response system (lexA/recA). SulA protein has been hypothesized to be additionally regulated proteolytically through the capR (lon) protease, i.e., in lon mutants lacking a functional ATP-dependent protease there would be more SulA protein. A hypothesized function for SulA protein is an inhibitor of cell septation. To investigate aspects of this model, we attempted to construct lon, lon sulA, and lon sulB strains containing multicopy plasmids specifying the sulA+ gene. Multicopy sulA+ plasmids could not be established in lon strains because more SulA protein accumulates than in a lon+ strain. When the sulA gene was mutated by a mini Mu transposon the plasmid could be established in the lon strains. In contrast, sulA+ plasmids could be established in lon+, lon sulA, and lon sulB strains. The sulA+ plasmids caused lon sulA and lon sulB cells to exist as filaments without SOS induction and to be sensitive to UV light and nitrofurantoin. Evidence implicated higher basal levels of SulA protein in these lon plasmid sulA+ strains as the cause of filamentation. We confirmed that the SulA protein is an 18-kilodalton polypeptide and demonstrated that it was induced by treatment with nalidixic acid. The SulA protein was rapidly degraded in a lon+ strain, but was comparatively more stable in vivo in a lon sulB mutant. Furthermore, the SulA protein was localized to the membrane by several techniques.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics,metabolism Cell Division Cell Membrane/analysis Escherichia coli/cytology,genetics,metabolism Genes, Bacterial Mutation Nalidixic Acid/pharmacology Operon Plasmids
Chemicals
Bacterial Proteins Nalidixic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schoemaker J M
Gayda R C
Markovitz A
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56 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.2.551-561.1984
Published
1984-05-00
Pages
551-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215464
Subset
IM
Grants
NIAID NIH HHS · AI 06966 · United States
NIGMS NIH HHS · GM 07543 · United States
External Links
PubMed source DOI: 10.1128/jb.158.2.551-561.1984 PMC Full Text View journal details
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