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PMID: 6328006 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence and host La protein interactions of rabies virus leader RNA.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 773-8

Kurilla MG, Cabradilla CD, Holloway BP, Keene JD

Abstract

Rabies virus leader RNA was detected in infected BHK-21 cell extracts by hybridization to end-labeled genomic RNA. Similar to the leader RNA of vesicular stomatitis virus, the leader RNA of rabies virus was also found to be associated with the La protein by specific immunoprecipitation with antisera from lupus patients. The 3' end of the genomic RNA of rabies virus was sequenced, and the size and termination site of leader RNA were determined. In addition, extension of the sequence into the nucleocapsid gene of rabies virus showed an open reading frame for at least 37 amino acid residues. Sequence relationships between rabies virus and vesicular stomatitis virus leader genes and the possible involvement of the La protein in rhabdovirus biology are discussed.

MeSH Terms
Animals Antigens/genetics Autoantigens Base Sequence Cell Line Cricetinae Genes, Viral Kidney Nucleic Acid Hybridization Protein Binding RNA, Viral/genetics Rabies virus/genetics Ribonucleoproteins Species Specificity Transcription Factors/genetics Vesicular stomatitis Indiana virus/genetics
Chemicals
Antigens Autoantigens RNA, Viral Ribonucleoproteins SS-B antigen Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kurilla M G
Cabradilla C D
Holloway B P
Keene J D
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40 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.773-778.1984
Published
1984-06-00
Pages
773-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255736
Subset
IM
Grants
NIGMS NIH HHS · GMO7171 · United States
NIAID NIH HHS · R01AI16099 · United States
Databases
GENBANK
M12771
External Links
PubMed source DOI: 10.1128/JVI.50.3.773-778.1984 PMC Full Text View journal details
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