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PMID: 6329683 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutant din-21, a variant of polyoma virus containing a mouse DNA sequence in the viral genome.

The EMBO journal ·Vol. 1 ·No. 4 ·1982-00-00 ·Pages 461-6

Ding D, Jones MD, Leigh-Brown A, Griffin BE

Abstract

An unusual non-defective mutant of polyoma virus with an anomalously large genome, designated din-21, has been isolated. The viral chromosome lacks 49 base pairs of the putative control region between the origin of replication and the initiation codon for the early proteins, the T-antigens. In their stead , 95 base pairs, with limited homology to the deleted sequence and apparently of mouse origin, have been inserted. The primary sequence of the insert DNA has been determined and some of the biological properties of the mutant examined. It transforms rat-1 cells slightly better than wild-type virus and grows slightly less well in lytically infected mouse cells. It does not interfere with the growth of wild-type polyoma virus. The properties of this mutant suggest that it is a natural isolate of mouse cells. The mutant was presumably generated by reciprocal recombination between polyoma DNA and mouse host DNA. This could be associated with the integration of a viral DNA sequence into the host chromosome during the viral replicative cycle.

MeSH Terms
Base Sequence Chromosome Deletion DNA Restriction Enzymes DNA Transposable Elements Genes Genes, Viral Genetic Variation Mutation Nucleic Acid Hybridization Polyomavirus/genetics Viral Plaque Assay
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ding D
Jones M D
Leigh-Brown A
Griffin B E
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
461-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553068
Subset
IM
Databases
GENBANK
K01705
External Links
PubMed source PMC Full Text View journal details
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