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PMID: 6344018 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

CAP binding to B and Z forms of DNA.

Nucleic acids research ·Vol. 11 ·No. 8 ·1983-04-25 ·Pages 2479-94

Fried MG, Wu HM, Crothers DM

Abstract

We have examined the interaction between the cyclic AMP receptor protein (CAP) and a small DNA fragment containing its specific recognition sequence by circular dichroism spectroscopy. The binding of CAP to this fragment induces a B to "C-like" change in the CD spectrum, which is different from that observed for non-specific binding. A one-to-one (CAP dimer to DNA) binding stoichiometry was deduced from spectroscopic titration data, as was a non-specific binding site size of 17 bp/dimer. In addition, we have compared the non-specific binding affinity of CAP for the B and Z forms of synthetic DNA copolymers. A slight preference for the B form was found. These results do not support the recent specific suggestion that CAP binds to a left-handed form of DNA (1), but indicate more generally that an optically detectable conformational change takes place in DNA on binding CAP.

MeSH Terms
Base Composition Chromosomes, Bacterial Circular Dichroism DNA DNA, Bacterial/genetics Escherichia coli/genetics Nucleic Acid Conformation Protein Binding Protein Conformation Receptors, Cyclic AMP/genetics
Chemicals
DNA, Bacterial Receptors, Cyclic AMP DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fried M G
Wu H M
Crothers D M
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.8.2479
Published
1983-04-25
Pages
2479-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325897
Subset
IM
Grants
NIGMS NIH HHS · GM-21966 · United States
External Links
PubMed source DOI: 10.1093/nar/11.8.2479 PMC Full Text View journal details
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