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PMID: 6361492 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Increased expression of the dnaA gene has no effect on DNA replication in a dnaA+ strain of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 192 ·No. 3 ·1983-00-00 ·Pages 506-8

Churchward G, Holmans P, Bremer H

Abstract

We have constructed a pBR322 plasmid derivative which expresses dnaA protein under the control of the E. coli lac UV5 promotor. Expression of the dnaA protein from the plasmid is inducible by isopropyl-beta-D-thiogalactoside. In a dnaA+ strain induction has no effect on the accumulation of DNA. In contrast, in a thermosensitive dnaA46 strain, induction, at either the permissive or the nonpermissive temperature, results in an immediate stimulation of DNA accumulation. We conclude that, while in a dnaA46 strain dnaA protein limits DNA replication, in a dnaA+ strain dnaA protein activity does not control the timing of replication initiation.

MeSH Terms
Bacterial Proteins/genetics Cloning, Molecular DNA Replication Escherichia coli/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Churchward G
Holmans P
Bremer H
References (21)
21 references, click to expand
  1. Chloramphenicol releases a block in initiation of chromosome replication in a dnaA strain of Escherichia coli K12.
    Mol Gen Genet. 1980;178(2):329-36 PMID: 6156379
  2. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.
    Mol Gen Genet. 1979 Sep;175(2):135-44 PMID: 160000
  3. Enzymatic replication of the origin of the Escherichia coli chromosome.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12 ):7370-4 PMID: 6278471
  4. The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
    Nucleic Acids Res. 1982 Nov 25;10(22):7373-85 PMID: 6296774
  5. Lac repressor and lac operator.
    Prog Biophys Mol Biol. 1975;30(2-3):227-52 PMID: 792953
  6. Suppression of the DnaA phenotype by mutations in the rpoB cistron of ribonucleic acid polymerase in Salmonella typhimurium and Escherichia coli.
    J Bacteriol. 1977 May;130(2):577-82 PMID: 400782
  7. Bacteriophage lambda and plasmid vectors, allowing fusion of cloned genes in each of the three translational phases.
    Nucleic Acids Res. 1978 Dec;5(12):4479-94 PMID: 370770
  8. The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
    EMBO J. 1982;1(9):1043-8 PMID: 6329723
  9. A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature.
    Mol Gen Genet. 1978 Jun 1;162(1):9-16 PMID: 353526
  10. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.
    J Mol Biol. 1971 Feb 14;55(3):441-56 PMID: 4927942
  11. Essential role of the gyrB gene product in the transcriptional event coupled to dnaA-dependent initiation of Escherichia coli chromosome replication.
    Mol Gen Genet. 1981;183(1):134-8 PMID: 6276673
  12. Continuous synthesis of the dnaA gene product of Escherichia coli in the cell cycle.
    Mol Gen Genet. 1982;186(1):87-94 PMID: 6287172
  13. Revised sequence of the tetracycline-resistance gene of pBR322.
    Gene. 1983 May-Jun;22(2-3):277-80 PMID: 6307828
  14. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein.
    J Bacteriol. 1977 Feb;129(2):1020-33 PMID: 320174
  15. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  16. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  17. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  18. Temporal sequence of events during the initiation process in Escherichia coli deoxyribonucleic acid replication: roles of the dnaA and dnaC gene products and ribonucleic acid polymerase.
    J Bacteriol. 1977 Mar;129(3):1466-75 PMID: 321429
  19. Regulation of the dnaA product in Escherichia coli.
    Mol Gen Genet. 1977 Oct 20;155(2):219-25 PMID: 337131
  20. The initiation of chromosome replication in a dnaAts46 and a dnaA+ strain at various temperatures.
    Mol Gen Genet. 1981;182(2):364-6 PMID: 7026978
  21. Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.
    Cold Spring Harb Symp Quant Biol. 1968;33:677-93 PMID: 4892005
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00392197
Published
1983-00-00
Pages
506-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM25948 · United States
External Links
PubMed source DOI: 10.1007/BF00392197
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