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PMID: 6366517 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.

Molecular and cellular biology ·Vol. 4 ·No. 2 ·1984-02-00 ·Pages 268-75

Laughon A, Driscoll R, Wills N, Gesteland RF

Abstract

We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.

MeSH Terms
Base Sequence Culture Media Fungal Proteins/biosynthesis,genetics Galactose/metabolism Genes, Fungal Operon Plasmids Protein Biosynthesis RNA, Fungal/isolation & purification Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic
Chemicals
Culture Media Fungal Proteins RNA, Fungal Galactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Laughon A
Driscoll R
Wills N
Gesteland R F
References (35)
35 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.2.268-275.1984
Published
1984-02-00
Pages
268-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368691
Subset
IM
Grants
NIGMS NIH HHS · GM07531 · United States
Databases
GENBANK
K01485
External Links
PubMed source DOI: 10.1128/mcb.4.2.268-275.1984 PMC Full Text View journal details
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