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PMID: 6371045 Published · ppublish English Journal Article

Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin.

Journal of clinical microbiology ·Vol. 19 ·No. 3 ·1984-03-00 ·Pages 394-8

Surujballi OP, Fackrell HB

Abstract

A sandwich enzyme-linked immunosorbent assay was developed for measuring Staphylococcus aureus alpha-toxin. This assay was 500 to 1,000 times more sensitive than the commonly used hemolytic titration assay and was less variable. The binding of alpha-toxin to the adsorbed antibody was most effective after an overnight incubation at 27 degrees C. The toxin was detectable even at a log2 17 dilution of an S. aureus culture supernatant.

MeSH Terms
Bacterial Toxins/analysis Enzyme-Linked Immunosorbent Assay Hemolysin Proteins Hemolytic Plaque Technique Immunoenzyme Techniques Staphylococcal Protein A/pharmacology Staphylococcus aureus/pathogenicity
Chemicals
Bacterial Toxins Hemolysin Proteins Staphylococcal Protein A staphylococcal alpha-toxin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Surujballi O P
Fackrell H B
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29 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.19.3.394-398.1984
Published
1984-03-00
Pages
394-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271072
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.19.3.394-398.1984 PMC Full Text View journal details
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