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PMID: 6429 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of Klebsiella aerogenes.

Journal of bacteriology ·Vol. 127 ·No. 1 ·1976-07-00 ·Pages 141-8

Bender RA, Macaluso A, Magasanik B

Abstract

The gene for glutamate dehydrogenase (gdhD) has been mapped in Klebsiella aerogenes by P1 transduction. It is linked to pyrF and trp with the order pyrF-trp-gdh. Complementation analysis using F' episomes from Escherichia coli suggests an analogous location in E. coli. Two mutants able to produce glutamate dehydrogenase in the presence of high levels of glutamine synthetase have been isolated. One, tightly linked to gdhD, shows normal repression control by glutamine synthetase but produces four times as much glutamate dehydrogenase activity as does the wild type under all conditions tested. The other revertant is not linked to gdhD or glnA.

MeSH Terms
Ammonia/metabolism Cell-Free System Chromosome Mapping Enterobacter/enzymology,metabolism Enterobacteriaceae/enzymology Enzyme Repression Escherichia coli/enzymology Ethyl Methanesulfonate Genes Genetic Linkage Glucose/metabolism Glutamate Dehydrogenase/biosynthesis,metabolism Glutamate Synthase/metabolism Glutamate-Ammonia Ligase/metabolism Glutamates/metabolism Mutagens Mutation Transduction, Genetic
Chemicals
Glutamates Mutagens Ammonia Ethyl Methanesulfonate Glutamate Synthase Glutamate Dehydrogenase Glutamate-Ammonia Ligase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bender R A
Macaluso A
Magasanik B
References (18)
18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.127.1.141-148.1976
Published
1976-07-00
Pages
141-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233044
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.127.1.141-148.1976 PMC Full Text View journal details
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