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PMID: 6453533 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Experimental diabetic autonomic neuropathy.

The American journal of pathology ·Vol. 103 ·No. 2 ·1981-05-00 ·Pages 210-25

Schmidt RE, Nelson JS, Johnson EM

Abstract

The regular occurrence of autonomic neuropathy, colonic dilatation, and loss of fecal consistency was investigated in streptozotocin-diabetic, age-matched control, and pancreatic-islet--transplanted rats using ultrastructural, histochemical, and biochemical methods. Degenerating unmyelinated axons were observed by electron microscopy in the colonic submucosa and muscularis, ileal mesentery, and splenic pedicle in 5--7 months diabetic animals; similar changes were not found in control rats or animals subjected to islet transplantation three weeks after induction of diabetes and sacrificed 4--6 months later (colon only). Regenerative changes, including axons with identifiable growth cones, were demonstrated in the mesenteric nerves of chronically diabetic animals. Formaldehyde-induced catecholamine fluorescence and cholinesterase histochemistry suggested deficiencies in colonic adrenergic and cholinergic innervation; histochemical findings in islet-transplanted animals were comparable to those of untreated control animals. Biochemical measurements of the adrenergic and cholinergic nervous system marker enzymes dopamine-beta-hydroxylase and choline acetyltransferase, respectively, in colon and spleen confirm a deficit in adrenergic (colon and spleen) and cholinergic (colon) innervation in chronically diabetic animals.

MeSH Terms
Animals Autonomic Nervous System/pathology Choline O-Acetyltransferase/analysis Colon/enzymology,innervation Diabetes Mellitus, Experimental/enzymology,pathology Diabetic Neuropathies/enzymology,pathology Dopamine beta-Hydroxylase/analysis Ileum/enzymology Male Mesentery/innervation Rats Rats, Inbred Lew Rats, Inbred WF Spleen/innervation Streptozocin
Chemicals
Streptozocin Dopamine beta-Hydroxylase Choline O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schmidt R E
Nelson J S
Johnson E M
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46 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1981-05-00
Pages
210-25
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1903833
Subset
IM
Grants
NINDS NIH HHS · 732 NS06115-0 · United States
NIADDK NIH HHS · AM-19645 · United States
NHLBI NIH HHS · HL-20604 · United States
External Links
PubMed source PMC Full Text View journal details
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