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PMID: 6454004 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Bacteriophage phi X174-specific mRNA synthesis in cells deficient in termination factor rho activity.

Journal of virology ·Vol. 38 ·No. 1 ·1981-04-00 ·Pages 198-207

Hayashi MN, Hayashi M, Imai M

Abstract

A previous report (Hayashi et al., Proc. Natl. Acad. Sci. U.S.A. 73:3519-3523, 1976) indicated that in vivo bacteriophage phi X174 mRNA's terminate after genes J, F, G, and H. However, termination at these sites is not stringent. To determine whether termination of phi X174 transcription depends on rho factor activity, we introduced a temperature-sensitive rho mutation (nitA) into a phi X174-sensitive host cell line and determined termination sites in wild-type and nitA cells. We found that (i) normal phi X174 terminators were recognized in phi X174-infected nitA cells, (ii) the rho mutation relieved polar effects caused by nonsense mutations in the phage genome or by chloramphenicol treatment of the host cells, and (iii) polarity was not caused by premature termination of transcription at the site of the polar mutation. RNA synthesis continued beyond the site to the first rho-sensitive site.

MeSH Terms
Bacteriophage phi X 174/genetics Chloramphenicol/pharmacology Mutation RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Rho Factor/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
RNA, Messenger RNA, Viral Rho Factor Transcription Factors Chloramphenicol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayashi M N
Hayashi M
Imai M
References (30)
30 references, click to expand
  1. Electrophoretic characterization of phiX174-specific proteins.
    J Mol Biol. 1969 Sep 28;44(3):501-16 PMID: 4899475
  2. Hyperdegradation of proteins in Escherichia coli rho mutants.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1623-7 PMID: 377279
  3. Relief of polarity in E. coli by "suA".
    Nature. 1970 Apr 4;226(5240):28-31 PMID: 4907561
  4. The eight genes of bacteriophages phi X174 and S13 and comparison of the phage-specified proteins.
    J Mol Biol. 1970 Apr 28;49(2):521-6 PMID: 5447830
  5. Direction of translation in phage S13 as determined from the sizes of polypeptide fragments of nonsense mutants.
    Virology. 1971 Feb;43(2):352-5 PMID: 5543825
  6. Polarity induced by chloramphenicol and relief by suA.
    J Mol Biol. 1971 Jan 14;55(1):113-8 PMID: 4926972
  7. Regulation of lac transcription in antibiotic-treated E. coli.
    Nat New Biol. 1971 Mar 10;230(10):41-4 PMID: 4324113
  8. Isolation of phi X174 specific messenger ribonucleic acids in vivo and identification of their 5' terminal nucleotides.
    J Virol. 1972 Feb;9(2):207-15 PMID: 4552415
  9. A promoter site and polarity gradients in phage S13.
    Virology. 1972 Oct;50(1):171-9 PMID: 4563038
  10. Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I.
    J Virol. 1974 Nov;14(5):1142-51 PMID: 4610178
  11. Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.
    Biochemistry. 1975 Jan 14;14(1):69-78 PMID: 1109593
  12. Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells.
    J Virol. 1975 Jan;15(1):151-60 PMID: 1089800
  13. Isolation and genetic characterization of the nitA mutants of Escherichia coli affecting the termination factor rho.
    Mol Gen Genet. 1976 Jan 16;143(2):211-21 PMID: 765755
  14. The effect of the termination rho factor and ribonuclease III on the transcription of bacteriophage phi X174 DNA in vitro.
    FEBS Lett. 1976 Apr 15;64(1):81-4 PMID: 773703
  15. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959-63 PMID: 132662
  16. The in vitro transcription units of bacteriophage phiX174. I. Characterization of synthetic parameters and measurement of transcript molecular weights.
    J Mol Biol. 1976 Jun 5;103(4):681-97 PMID: 781282
  17. The in vitro transcription units of bacteriophage phiX174. II. In vitro initiation sites of phiX174 transcription.
    J Mol Biol. 1976 Jun 5;103(4):699-710 PMID: 781283
  18. The in vitro transcription units of bacteriophage phiX174. III. Initiation with specific 5' end oligonucleotides of in vitro phiX174 RNA.
    J Mol Biol. 1976 Jun 5;103(4):711-35 PMID: 781284
  19. Mapping of in vivo messenger RNAs for bacteriophage phiX-174.
    Proc Natl Acad Sci U S A. 1976 Oct;73(10):3519-23 PMID: 1068463
  20. Transcription of bacteriophage phi-X174 in vitro: selective initiation with oligonucleotides.
    J Mol Biol. 1976 Dec 25;108(4):753-70 PMID: 1018323
  21. Transcription of bacteriophage phiX174 in vitro: analysis with restriction enzymes.
    J Mol Biol. 1976 Dec 25;108(4):771-9 PMID: 1018324
  22. Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1162-6 PMID: 322147
  23. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  24. Sequences and efficiencies of proposed mRNA terminators.
    Nature. 1978 Jan 19;271(5642):275-7 PMID: 622168
  25. Studies on the altered rho factor in a nitA mutants of Escherichia coli defective in transcription termination. I. Characterization and quantitative determination of rho in cell extracts.
    J Mol Biol. 1978 Apr 25;120(4):451-66 PMID: 148514
  26. Studies on the altered rho factor in nitA mutants of Escherichia coli defective in transcription termination. II. Purification and molecular properties of the mutant rho.
    J Mol Biol. 1978 Apr 25;120(4):467-86 PMID: 148515
  27. Control of transcription termination.
    Annu Rev Biochem. 1978;47:967-96 PMID: 354508
  28. The nucleotide sequence of bacteriophage phiX174.
    J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
  29. Effect of Escherichia coli rho factor and RNase III on the formation of phi X174 RNA in vitro.
    FEBS Lett. 1979 Feb 1;98(1):123-7 PMID: 428528
  30. Termination factor for RNA synthesis.
    Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.1.198-207.1981
Published
1981-04-00
Pages
198-207
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171140
Subset
IM
Grants
NIGMS NIH HHS · GM 12934 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.1.198-207.1981 PMC Full Text View journal details
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