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PMID: 662692 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells.

Nucleic acids research ·Vol. 5 ·No. 5 ·1978-05-00 ·Pages 1515-28

Detke S, Stein JL, Stein GS

Abstract

Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.

MeSH Terms
Amanitins/pharmacology Cell Cycle Cell Nucleus/enzymology DNA-Directed RNA Polymerases/metabolism HeLa Cells/enzymology Histones/biosynthesis Kinetics Nucleic Acid Hybridization RNA Polymerase II/metabolism RNA, Messenger/biosynthesis Transcription, Genetic/drug effects
Chemicals
Amanitins Histones RNA, Messenger RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Detke S
Stein J L
Stein G S
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50 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.5.1515
Published
1978-05-00
Pages
1515-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342100
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.5.1515 PMC Full Text View journal details
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