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PMID: 6687940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.

Nucleic acids research ·Vol. 11 ·No. 8 ·1983-04-25 ·Pages 2325-35

Jaye M, de la Salle H, Schamber F, Balland A, Kohli V, Findeli A, Tolstoshev P, Lecocq JP

Abstract

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images

MeSH Terms
Alanine Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular Codon/genetics DNA/isolation & purification Factor IX/genetics Hemophilia A/genetics Humans Liver/metabolism Oligodeoxyribonucleotides Oligonucleotides Species Specificity
Chemicals
Codon Oligodeoxyribonucleotides Oligonucleotides Factor IX DNA Alanine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Jaye M
de la Salle H
Schamber F
Balland A
Kohli V
Findeli A
Tolstoshev P
Lecocq J P
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.8.2325
Published
1983-04-25
Pages
2325-35
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325887
Subset
IM
Databases
GENBANK
J00137, J02523
External Links
PubMed source DOI: 10.1093/nar/11.8.2325 PMC Full Text View journal details
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