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PMID: 6695923 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of repetitive DNA sequences from human chromosome 21.

American journal of human genetics ·Vol. 36 ·No. 1 ·1984-01-00 ·Pages 25-35

Graham GJ, Hall TJ, Cummings MR

Abstract

We have developed a method for the isolation of phage from the human genomic library that carry repetitive DNA sequences highly represented on specific human chromosomes. We have used this technique to select recombinants carrying inserts concentrated on chromosome 21. Five clones, representing two families of sequences, have been characterized. Members of each family show cross-homology, but the two families show no homology with each other. In all but one case, the clones do not contain members of the human Alu repeat family. Single chromosome-concentrated repetitive sequences should prove to be useful in studies of the origin, evolution, and function of repetitive DNA and in regional chromosome mapping.

MeSH Terms
Animals Chromosome Mapping Chromosomes, Human, 21-22 and Y Clone Cells Cricetinae DNA/analysis,genetics DNA, Recombinant/analysis Humans Hybrid Cells Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Graham G J
Hall T J
Cummings M R
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32 references, click to expand
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1984-01-00
Pages
25-35
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684372
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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