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PMID: 6712631 Published · ppublish English Journal Article

Antizyme to ornithine decarboxylase is present in the liver of starved rats.

The Biochemical journal ·Vol. 218 ·No. 2 ·1984-03-01 ·Pages 557-62

Fujita K, Matsufuji S, Murakami Y, Hayashi S

Abstract

Antizyme to ornithine decarboxylase (ODC) and ODC-antizyme complex were both present in liver cytosols of starved rats. The antizyme was identified by its molecular weight, kinetic properties, formation of a complex with ODC, and reversal of its inhibition by antizyme inhibitor. The average amount of antizyme in liver cytosols of starved rats was 0.1 unit/mg of protein, roughly corresponding to basal hepatic ODC activity in rats fed ad libitum. The presence of ODC-antizyme complex was detected by using antizyme inhibitor. These results indicate that antizyme participates in the regulation of ODC activity in vivo under physiological conditions.

MeSH Terms
Animals Chromatography, Gel Cytosol/enzymology In Vitro Techniques Kinetics Liver/enzymology Macromolecular Substances Male Molecular Weight Ornithine Decarboxylase Inhibitors Proteins/pharmacology Rats Rats, Inbred Strains Starvation/enzymology
Chemicals
Macromolecular Substances Ornithine Decarboxylase Inhibitors Proteins ornithine decarboxylase antizyme
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fujita K
Matsufuji S
Murakami Y
Hayashi S
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25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2180557
Published
1984-03-01
Pages
557-62
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153372
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2180557 PMC Full Text View journal details
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