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PMID: 67172 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The monoclonal anti-phosphorylcholine antibody response in several murine strains: genetic implications of a diverse repertoire.

The Journal of experimental medicine ·Vol. 145 ·No. 4 ·1977-04-01 ·Pages 876-91

Gearhart PJ, Sigal NH, Klinman NR

Abstract

The idiotypic identification of monoclonal antibodies has been used to define and enumerate clonotypes within the murine repertoire of B cells specific for phosphorylcholine (PC). The response in the BALB/c strain is dominated by a single antibody specificity which is identical to TEPC 15 protein; however, antibody without the TEPC 15 idiotype appears heterogeneous by idiotypic cross-reactivity and hapten inhibition of binding to antigen. Dissection of the PC-specific repertoire in the AKR, A/He, and C3H strains has indicated that some monoclonal antibodies share binding-site idiotypic determinants with TEPC 15, although these clones represent a minority of the precursor cells. In addition to providing insights into the heterogeneity and expression of the murine B-cell repertoire, these studies emphasize structural relationships between PC-specific clonotypes. Within the BALB/c strain, some antibodies share combining-site-related idiotypic specificities with TEPC 15, but differ in other variable region determinants. Among allotypically distinct strains, there exists a remarkable similarity of variable region determinants in at least a minority of antibodies.

MeSH Terms
Animals Antibody Formation Antibody Specificity Binding Sites, Antibody Choline/analogs & derivatives Epitopes Genes Immunoglobulin Allotypes Mice Phosphorylcholine/immunology
Chemicals
Epitopes Immunoglobulin Allotypes Phosphorylcholine Choline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gearhart P J
Sigal N H
Klinman N R
References (27)
27 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.145.4.876
Published
1977-04-01
Pages
876-91
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180641
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.145.4.876 PMC Full Text View journal details
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