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PMID: 6761650 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The regulatory region of the divergent argECBH operon in Escherichia coli K-12.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8031-48

Piette J, Cunin R, Boyen A, Charlier D, Crabeel M, Van Vliet F, Glansdorff N, Squires C, Squires CL

Abstract

The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding sites. A long leader sequence - not involved in attenuation - precedes argCBH. Overlapping of the argCBH promoter and the region involved in ribosome mobilization for argE translation explains the dual effect of some mutations. Mutations causing semi-constitutive expression of argE improve putative promoter sequences within argC. Implications of these results regarding control mechanisms in amino acid biosynthesis and their evolution are discussed.

MeSH Terms
Base Sequence Biological Evolution Escherichia coli/genetics Genes, Bacterial Genes, Regulator Mutation Operon Plasmids Protein Biosynthesis Transcription, Genetic
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Piette J
Cunin R
Boyen A
Charlier D
Crabeel M
Van Vliet F
Glansdorff N
Squires C
Squires C L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8031
Published
1982-12-20
Pages
8031-48
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327068
Subset
IM
Databases
GENBANK
J01587
External Links
PubMed source DOI: 10.1093/nar/10.24.8031 PMC Full Text View journal details
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