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PMID: 6777778 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 6027-31

Dolby TW, Devuono J, Croce CM

Abstract

Purified mRNAs coding for mu and kappa human immunoglobulin polypeptides were translated in vitro and their products were characterized. The mu-specific mRNAs, derived from both human lymphoblastoid cells (GM607) and from a mouse-human somatic cell hybrid secreting human mu chains (alpha D5-H11-BC11), were copied into cDNAs and inserted into the plasmid pBR322. Several recombinant cDNAs that were obtained were identified by a combination of colony hybridization with labeled probes, in vitro translation of plasmid-selected mu mRNAs, and DNA nucleotide sequence determination. One recombinant DNA, for which the sequence has been partially determined, contains the codons for part of the C3 constant region domain through the carboxy-terminal piece (155 amino acids total) as well as the entire 3' noncoding sequence up to the poly(A) site of the human mu mRNA. The sequence A-A-U-A-A occurs 12 nucleotides prior to the poly(A) addition site in the human mu mRNA. Considerable sequence homology is observed in the mouse and human mu mRNA 3' coding and noncoding sequences.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Cell Line Cloning, Molecular DNA, Recombinant Genes Humans Hybrid Cells/immunology Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Immunoglobulin mu-Chains/genetics Mice Myeloma Proteins/immunology RNA, Messenger/genetics
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin kappa-Chains Immunoglobulin mu-Chains Myeloma Proteins RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dolby T W
Devuono J
Croce C M
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.10.6027
Published
1980-10-00
Pages
6027-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC350206
Subset
IM
Grants
NCI NIH HHS · CA16685 · United States
NCI NIH HHS · CA23568 · United States
NCI NIH HHS · CA25875 · United States
Databases
GENBANK
J00257, J00258
External Links
PubMed source DOI: 10.1073/pnas.77.10.6027 PMC Full Text View journal details
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