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PMID: 70785 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2840-3

Myers JC, Spiegelman S, Kacian DL

Abstract

The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.

MeSH Terms
Avian Leukosis Virus/metabolism Avian Myeloblastosis Virus/enzymology,metabolism Base Sequence DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Molecular Weight Nucleic Acid Hybridization RNA-Directed DNA Polymerase/metabolism Templates, Genetic
Chemicals
DNA, Single-Stranded DNA, Viral RNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Myers J C
Spiegelman S
Kacian D L
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2840
Published
1977-07-00
Pages
2840-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431313
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2840 PMC Full Text View journal details
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