Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.

Journal of clinical microbiology ·Vol. 13 ·No. 3 ·1981-03-00 ·Pages 449-55

Mackie EB, White LA, Brown KN, Bryan LE

Abstract

Rabbit antisera generated against 11 prototype strains of group B Neisseria meningitidis were tritiated by using the enzyme-linked immunosorbent assay and the Ouchterlony immunoprecipitation technique. The relative insensitivity of the immunoprecipitation technique in detecting antibody compared with the enzyme-linked immunosorbent assay was demonstrated. Cross-reactions within the group and among the 11 serotypes were characterized with respect to established serological techniques. Serotype polymorphism among the strains of N. meningitidis was demonstrated with the enzyme-linked immunosorbent assay.

MeSH Terms

Agglutination Tests Antibodies, Bacterial/analysis Antigens, Bacterial Cross Reactions Enzyme-Linked Immunosorbent Assay Immunodiffusion Neisseria meningitidis/classification,immunology Polymorphism, Genetic Serotyping/methods

Chemicals

Antibodies, Bacterial Antigens, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mackie E B

White L A

Brown K N

Bryan L E

References (24)

24 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.13.3.449-455.1981

Published

1981-03-00

Pages

449-55

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC273812

Subset

External Links

PubMed source DOI: 10.1128/jcm.13.3.449-455.1981 PMC Full Text View journal details

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Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.

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