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PMID: 6787214 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivatives of Pseudomonas aeruginosa strain PAO.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 529-38

Jarrell KF, Kropinski AM

Abstract

A lipopolysaccharide (LPS)-defective (rough) mutant of Pseudomonas aeruginosa PAO was isolated by selection for resistance to the LPS-specific phage E79. The LPS of this mutant, AK-1012, lacked the O-antigenic side chain-specific amino sugar fucosamine as well as the core-specific sugars glucose and rhamnose. Using this strain, we isolated and characterized a phage, phi PLS27, which is specifically inactivated upon incubation with LPS extracted from rough mutants of P. aeruginosa PAO. phi PLS27 was found to be a Bradley type C phage and was very similar to coliphage T7 in a number of properties, including size, buoyant density, mass, and the number of structural proteins.

MeSH Terms
Bacteriophages/isolation & purification,physiology,ultrastructure Centrifugation, Density Gradient DNA, Viral/analysis Deoxycholic Acid/pharmacology Lipopolysaccharides/pharmacology Pseudomonas aeruginosa/physiology Viral Proteins/analysis
Chemicals
DNA, Viral Lipopolysaccharides Viral Proteins Deoxycholic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jarrell K F
Kropinski A M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.529-538.1981
Published
1981-05-00
Pages
529-38
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171183
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.38.2.529-538.1981 PMC Full Text View journal details
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