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PMID: 6813310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Permeability of Pseudomonas aeruginosa outer membrane to hydrophilic solutes.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 636-42

Yoshimura F, Nikaido H

Abstract

Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydrolysis of cephacetrile, cephaloridine, and various phosphate esters by hydrolytic enzymes located in the periplasm. The permeability to these compounds was about 100-fold lower than in the outer membrane of Escherichia coli K-12. Also, we found that the apparent Km values for active transport of various carbon and energy source compounds were typically higher than 20 microM in P. aeruginosa, in contrast to E. coli in which the values are usually lower than 5 microM. These results also are consistent with the notion that the P. aeruginosa outer membrane indeed has a low permeability to most hydrophilic compounds and that this membrane acts as a rate limiting step in active transport processes with high Vmax values.

MeSH Terms
Cell Membrane Permeability Cephacetrile/metabolism Cephaloridine/metabolism Escherichia coli/metabolism Kinetics Pseudomonas aeruginosa/growth & development,metabolism Solutions Species Specificity Spheroplasts/metabolism
Chemicals
Solutions Cephacetrile Cephaloridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yoshimura F
Nikaido H
References (32)
32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.636-642.1982
Published
1982-11-00
Pages
636-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221510
Subset
IM
Grants
NIAID NIH HHS · AI-09644 · United States
External Links
PubMed source DOI: 10.1128/jb.152.2.636-642.1982 PMC Full Text View journal details
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