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PMID: 6819544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of human variable and constant region immunoglobulin heavy chain genes on subtelomeric band q32 of chromosome 14.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8155-70

McBride OW, Battey J, Hollis GF, Swan DC, Siebenlist U, Leder P

Abstract

Analysis of a group of human/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human variable region (VH), junctional (JH), and constant region (C epsilon) heavy chain immunoglobulin genes indicates that all of these IgH genes are localized on the subtelomeric (q32) band of chromosome 14. Somatic cell hybrids were isolated in selective medium after fusing human fibroblasts with hprt- Chinese hamster cells. The human parental cells contained two translocation chromosomes representing a reciprocal translocation between chromosomes X and 14. Only those hybrid cell lines retaining a complete human autosome 14 or the X/14 translocation chromosome (i.e. containing band 14q32) retained the human IgH genes. Retention of these genes did not correlate with the presence of the other translocation chromosome, 14/X. These results indicate that all human IgH genes (VH, JH, and CH) map to the same chromosomal band (14q32) which is commonly involved in reciprocal translocations with human chromosome 8 (8q24) in B-cell neoplasms.

MeSH Terms
Animals Binding Sites, Antibody/genetics Cell Line Chromosomes, Human, 13-15 Cricetinae Cricetulus Fibroblasts/metabolism Genes Humans Hybrid Cells Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulins/genetics Karyotyping Translocation, Genetic
Chemicals
Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Immunoglobulin Variable Region Immunoglobulins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
McBride O W
Battey J
Hollis G F
Swan D C
Siebenlist U
Leder P
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8155
Published
1982-12-20
Pages
8155-70
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327076
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.24.8155 PMC Full Text View journal details
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