Home LiteratureArticle Details
PMID: 6928686 Published · ppublish English Journal Article

Reversible dissociation of linker histone from chromatin with preservation of internucleosomal repeat.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 885-9

Allan J, Staynov DZ, Gould H

Abstract

Procedures are described for the dissociation of histones H1 and H5 from chicken reticulocyte chromatin without disruption of the native core histone-DNA complex. The comparative properties of native and depleted chromatin with respect to sedimentation, thermal denaturation, and sensitivity to nuclease digestion have been studied. The changes in these properties resulting from removal of the linker histones are fully reversed when histone H5 is added back to the depleted chromatin.

MeSH Terms
Animals Chickens Chromatin/metabolism,ultrastructure Histones/metabolism Hot Temperature Micrococcal Nuclease/metabolism Molecular Weight Nucleic Acid Denaturation Nucleosomes/ultrastructure Reticulocytes/ultrastructure
Chemicals
Chromatin Histones Nucleosomes Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Allan J
Staynov D Z
Gould H
References (43)
43 references, click to expand
  1. Action of micrococcal nuclease on chromatin and the location of histone H1.
    J Mol Biol. 1977 Jan 25;109(3):393-404 PMID: 833849
  2. Heterogeneity of chromatin subunits in vitro and location of histone H1.
    Nucleic Acids Res. 1976 Feb;3(2):477-92 PMID: 1257057
  3. A colorimetric method for the determination of submicrogram quantities of protein.
    Anal Biochem. 1977 Mar;78(1):86-92 PMID: 848760
  4. Involvement of histone H1 in the organization of the chromosome fiber.
    Proc Natl Acad Sci U S A. 1977 May;74(5):1879-83 PMID: 266711
  5. Specific folding and contraction of DNA by histones H3 and H4.
    Cell. 1977 Jul;11(3):609-18 PMID: 195743
  6. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  7. Influence of histone H1 on chromatin structure.
    Cell. 1977 Sep;12(1):101-7 PMID: 561660
  8. Higher order coiling of DNA in chromatin.
    Cell. 1977 Sep;12(1):83-100 PMID: 409504
  9. A nucleosome-like structure containing DNA and the arginine-rich histones H3 and H4.
    Nucleic Acids Res. 1977 Jul;4(7):2477-85 PMID: 561948
  10. Reconstitution of chromatin core particles.
    Biochemistry. 1977 Nov 29;16(24):5295-303 PMID: 921932
  11. Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin.
    Eur J Biochem. 1978 Feb;83(2):615-28 PMID: 631138
  12. Higher order structure of simian virus 40 chromatin.
    Science. 1978 Aug 4;201(4354):406-15 PMID: 208155
  13. The assay of globin gene transcription in reconstituted chromatin.
    Methods Cell Biol. 1978;19:387-422 PMID: 692420
  14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  15. Interaction of histone half-molecules with deoxyribonucleic acid.
    Biochemistry. 1971 Apr 13;10(8):1461-70 PMID: 5580664
  16. Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA.
    Eur J Biochem. 1971 Sep 24;22(2):235-45 PMID: 5116611
  17. Chromatin structure: a repeating unit of histones and DNA.
    Science. 1974 May 24;184(4139):868-71 PMID: 4825889
  18. Role of histones in chromatin condensation.
    Eur J Biochem. 1974 Dec 2;49(3):477-84 PMID: 4442423
  19. Electron-microscope observations on the organization of the nucleus in chicken erythrocytes and a superunit thread hypothesis for chromosome structure.
    J Cell Sci. 1974 Nov;16(2):261-99 PMID: 4141361
  20. Nucleic acids attached to solid matrices.
    Methods Enzymol. 1974;34:463-75 PMID: 4141407
  21. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
    Cell. 1975 Apr;4(4):281-300 PMID: 1122558
  22. Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. Histone H1 in chromatin and in H1 - DNA complexes.
    Eur J Biochem. 1975 Sep 1;57(1):97-105 PMID: 1175645
  23. Analysis of subunit organization in chicken erythrocyte chromatin.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):505-9 PMID: 1061151
  24. Physical studies of chromatin. The recombination of histones with DNA.
    Eur J Biochem. 1976 Feb 2;62(1):21-31 PMID: 1248482
  25. THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI.
    Can J Biochem. 1964 Dec;42:1743-52 PMID: 14241608
  26. Solenoidal model for superstructure in chromatin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901 PMID: 1064861
  27. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.
    Biochemistry. 1976 Jul 27;15(15):3307-14 PMID: 952859
  28. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
    Cell. 1976 Jul;8(3):333-47 PMID: 986252
  29. Chromatin structure as probed by nucleases and proteases: evidence for the central role of histones H3 and H4.
    Cell. 1976 Sep;9(1):179-93 PMID: 987855
  30. Nuclease digestion of reconstituted chromatin.
    FEBS Lett. 1976 Nov;70(1):67-70 PMID: 791679
  31. Thermal denaturation profiles and the structure of chromatin.
    Nature. 1976 Dec 9;264(5586):522-5 PMID: 1004587
  32. Stability of nucleosomes in native and reconstituted chromatins.
    Nucleic Acids Res. 1976 Nov;3(11):3173-92 PMID: 188018
  33. Subunit structure of simian-virus-40 minichromosome.
    Eur J Biochem. 1976 Nov 15;70(2):543-53 PMID: 188649
  34. The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones.
    Nucleic Acids Res. 1978 Oct;5(10):3479-89 PMID: 214759
  35. Analysis of chromatin reconstitutiion.
    Biochemistry. 1979 Feb 20;18(4):659-68 PMID: 420808
  36. An octamer of histones H3 and H4 forms a compact complex with DNA of nucleosome size.
    Nucleic Acids Res. 1978 Dec;5(12):4805-18 PMID: 745994
  37. Nucleosome packing in chromatin as revealed by nuclease digestion.
    Nucleic Acids Res. 1979 Jan;6(1):399-419 PMID: 370787
  38. Selective displacement of histone H1 from whole HeLa nuclei: effect on chromatin structure in situ as probed by micrococcal nuclease.
    Biochemistry. 1979 May 29;18(11):2160-6 PMID: 444446
  39. The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease.
    Nucleic Acids Res. 1979;6(5):1843-62 PMID: 450715
  40. Histone 1 is proximal to histone 2A and to A24.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2190-4 PMID: 287057
  41. Pathway-dependent reconstitution of chromatin structure from separated constituents.
    J Biol Chem. 1979 Nov 10;254(21):11061-5 PMID: 500624
  42. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  43. Reconstitution of chromatin subunits.
    Science. 1977 Mar 25;195(4284):1350-2 PMID: 841333
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.885
Published
1980-02-00
Pages
885-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348386
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.885 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2