Home LiteratureArticle Details
PMID: 6946479 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Repetitive DNA sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5386-90

Zuker C, Lodish HF

Abstract

We have isolated a clone of Dictyostelium discoideum genomic DNA, pB41-6, that contains sequences that are reiterated and interspersed in the genome. The expression of these reiterated sequences is developmentally regulated, and they appear to be linked only to developmentally regulated mRNAs. We have used clone pB41-6 to isolate a set of complementary cDNA clones. Analysis of two selected clones indicates that they encode developmentally regulated mRNA species and, by means of the repetitive elements they contain, hybridize to many developmentally regulated mRNAs. In addition, we have defined two families of mRNA species carrying these repetitive sequences: one is induced at 5.5 hr of development and the other is induced between 5.5 and 15 hr of development. Since most of the mRNA species that have sequences complementary to clone pB41-6 accumulate in a coordinated fashion during development, these sequences may play an important regulatory role in the developmental program of Dictyostelium.

MeSH Terms
Dictyostelium/genetics,growth & development Gene Expression Regulation Genetic Linkage Nucleic Acid Hybridization RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zuker C
Lodish H F
References (26)
26 references, click to expand
  1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  2. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  3. A role for cyclic AMP in expression of developmentally regulated genes in Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1044-8 PMID: 6244577
  4. Complexity of nuclear and polysomal RNAs in growing Dictyostelium discoideum cells.
    Dev Biol. 1980 Aug;78(2):268-84 PMID: 6157589
  5. Changes in the messenger RNA population during differentiation of dictyostelium discoideum.
    Dev Biol. 1980 Aug;78(2):285-300 PMID: 7409306
  6. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  7. Message sequences and short repetitive sequences are interspersed in sea urchin egg poly(A)+ RNAs.
    Nature. 1980 Sep 11;287(5778):111-7 PMID: 7191945
  8. Changes in the complexity of nuclear RNA during development of Dictyostelium discoideum.
    Dev Biol. 1981 Jan 15;81(1):74-80 PMID: 6161854
  9. Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition.
    Nucleic Acids Res. 1981 Feb 25;9(4):947-63 PMID: 7232208
  10. Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell--cell contact and cAMP.
    Cell. 1981 Jun;24(3):785-97 PMID: 6265103
  11. Gene regulation for higher cells: a theory.
    Science. 1969 Jul 25;165(3891):349-57 PMID: 5789433
  12. Temporal control of differentiation in the slime mold, Dictyostelium discoideum.
    Exp Cell Res. 1970 May;60(2):285-9 PMID: 5463774
  13. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  14. Structural organization of the genome of the cellular slime mold Dictyostelium discoideum: interspersion of repetitive and single-copy DNA sequences.
    Cell. 1975 Aug;5(4):401-11 PMID: 1171729
  15. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  16. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  17. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  18. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum.
    Dev Biol. 1977 Oct 1;60(1):180-206 PMID: 561722
  19. Synthesis of developmentally regulated proteins in Dictyostelium discoideum which are dependent on continued cell-cell interaction.
    Dev Biol. 1977 Oct 1;60(1):207-16 PMID: 561723
  20. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  21. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  22. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  23. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  24. Regulation of gene expression: possible role of repetitive sequences.
    Science. 1979 Jun 8;204(4397):1052-9 PMID: 451548
  25. A family of short, interspersed repeat sequences at the 5' end of a set of Dictyostelium single-copy mRNAs.
    Cell. 1979 Apr;16(4):787-96 PMID: 455448
  26. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.9.5386
Published
1981-09-00
Pages
5386-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348750
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.9.5386 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2