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PMID: 6949897 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Construction of a genetic map for Caulobacter crescentus.

Journal of bacteriology ·Vol. 149 ·No. 3 ·1982-03-00 ·Pages 889-96

Barrett JT, Rhodes CS, Ferber DM, Jenkins B, Kuhl SA, Ely B

Abstract

RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid recombinants are recovered at frequencies of 10(-6) and 10(-7) per donor cell. The data from five-factor crosses were subjected to computer-assisted crossover analyses as a rapid method to determine marker order. Using this information and data from additional two- and three-factor crosses mediated by RP4 or the generalized transducing bacteriophage phi Cr30, we constructed the first genetic map for C. crescentus.

MeSH Terms
Bacteria/genetics Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Genes, Bacterial Genetic Linkage Genetic Markers Transducers
Chemicals
Genetic Markers
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Barrett J T
Rhodes C S
Ferber D M
Jenkins B
Kuhl S A
Ely B
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.149.3.889-896.1982
Published
1982-03-00
Pages
889-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216475
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.149.3.889-896.1982 PMC Full Text View journal details
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