Antigen-specific T-helper cells stimulate H-2-compatible and H-2-incompatible B-cell blasts polyclonally.

The Journal of experimental medicine ·Vol. 151 ·No. 1 ·1980-01-01 ·Pages 194-203

Schreier MH, Andersson J, Lernhardt W, Melchers F

Abstract

Lipopolysaccharide (LPS)-activated B-cell blasts from C57BL/6J nu/nu spleen cells develop into IgM-secreting clones after stimulation by antigen-specific T-helper cells of C57BL/6J origin. Although induction of help is antigen-dependent, help itself acts polyclonally. 1 of 1--3 B-cell blasts is restimulated in a homologous fashion by LPS, or in a heterologous fashion by sheep erythrocyte (SRC)- or horse erythrocyte (HRC)-activated T-helper cells. The repertoire of activated B-cell blasts reflects the polyclonal nature of activation: approximately 1 in 1,000--3,000 restimulated B-cell blasts is specific for SRC, 1 in 300--1,000 is specific for HRC, and 1 in 100--300 specific for trinitrophenylated SRC (TNP30-SRC). B-cell blasts that are either H-2 compatible or H-2 incompatible with the antigen-activated T-cell help are stimulated polyclonally in similar high frequencies. Thus, neither antigen nor H-2 compatibility are required to stimulate a B-cell blast into the next cell cycle.

MeSH Terms

Animals Antibody Formation B-Lymphocytes/immunology Erythrocytes/immunology Histocompatibility Horses Lipopolysaccharides/immunology Lymphocyte Activation Mice Rats Sheep T-Lymphocytes/immunology Trinitrobenzenes

Chemicals

Lipopolysaccharides Trinitrobenzenes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schreier M H

Andersson J

Lernhardt W

Melchers F

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25 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.151.1.194

Published

1980-01-01

Pages

194-203

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2185768

Subset

External Links

PubMed source DOI: 10.1084/jem.151.1.194 PMC Full Text View journal details

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Antigen-specific T-helper cells stimulate H-2-compatible and H-2-incompatible B-cell blasts polyclonally.

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