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PMID: 6986354 Published · ppublish English Journal Article

Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.

Journal of bacteriology ·Vol. 141 ·No. 1 ·1980-01-00 ·Pages 121-8

Kiss GB, Dobo K, Dusha I, Breznovits A, Orosz L, Vincze E, Kondorosi A

Abstract

Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria.

MeSH Terms
Bacteriophages Chromosomes, Bacterial Conjugation, Genetic DNA, Bacterial/genetics Escherichia coli/genetics Genes R Factors Recombination, Genetic Rhizobium/genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kiss G B
Dobo K
Dusha I
Breznovits A
Orosz L
Vincze E
Kondorosi A
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.1.121-128.1980
Published
1980-01-00
Pages
121-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293544
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.141.1.121-128.1980 PMC Full Text View journal details
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