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PMID: 6991871 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The role of umuC gene product in mutagenesis by simple alkylating agents.

Molecular & general genetics : MGG ·Vol. 177 ·No. 4 ·1980-00-00 ·Pages 661-5

Schendel PF, Defais M

Abstract

This paper describes studies to determine the role of the umuC gene product in the process of alkylation induced mutagenesis. An active umuC gene is necessary for most MMS induced mutagenesis but it is not essential for EMS nor for MNNG induced mutagenesis in either normal or adapted cultures. In this respect the umuC mutation differs from lexA mutations which have a striking effect on MNNG induced mutagenesis (Schendel, et al., 1978). These findings have prompted a re-evaluation of these previously published data and the advancement of an hypothesis which explains the lexA effect without evoking a role for error-prone repair in the process of alkylation induced mutagenesis. It was also observed that exposure to MNNG is capable of generating a small amount of W-reactivation and W-mutagenesis capacity in a umuC strain which is totally blocked for UV induced reactivation. In light of this result a possible function for the umuC gene product is discussed.

MeSH Terms
Alkylating Agents/pharmacology DNA Repair/drug effects DNA, Bacterial/radiation effects Dose-Response Relationship, Drug Escherichia coli/genetics Ethyl Methanesulfonate/pharmacology Methyl Methanesulfonate/pharmacology Methylnitronitrosoguanidine/pharmacology Mutagens Mutation Ultraviolet Rays
Chemicals
Alkylating Agents DNA, Bacterial Mutagens Methylnitronitrosoguanidine Ethyl Methanesulfonate Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schendel P F
Defais M
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272677
Published
1980-00-00
Pages
661-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272677
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