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PMID: 7009584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Citrate-tris(hydroxymethyl)aminomethane-mediated release of outer membrane sections from the cell envelope of a deep-rough (heptose-deficient lipopolysaccharide) strain of Escherichia coli O8.

Journal of bacteriology ·Vol. 145 ·No. 3 ·1981-03-00 ·Pages 1386-96

Irvin RT, MacAlister TJ, Chan R, Costerton JW

Abstract

A heptose-deficient lipopolysaccharide strain of Escherichia coli O8, strain F515, was found to release portions of its outer membrane when cells were exposed to 10 mM citrate buffer (pH 2.75) for 30 min and subsequently exposed to 100 mM tris(hydroxymethyl)aminomethane buffer (pH 8.00). The outer membrane component release was found to be composed of protein, lipopolysaccharide, phospholipid (cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol), and alkaline phosphatase. The outer membrane component was released from the cell envelope in the absence of cell lysis, as no glucose-6-phosphate dehydrogenase activity or succinic dehydrogenase activity was detected. Morphologically, the outer membrane component appeared to consist of laminar fragments and vesicles which had an associated alkaline phosphatase activity.

MeSH Terms
Bacterial Proteins/analysis Cell Membrane/analysis,drug effects,ultrastructure Citrates/pharmacology Escherichia coli/drug effects,ultrastructure Heptoses/analysis Lipopolysaccharides/analysis Membrane Proteins/analysis Tromethamine/pharmacology
Chemicals
Bacterial Proteins Citrates Heptoses Lipopolysaccharides Membrane Proteins Tromethamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Irvin R T
MacAlister T J
Chan R
Costerton J W
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.3.1386-1396.1981
Published
1981-03-00
Pages
1386-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217143
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.145.3.1386-1396.1981 PMC Full Text View journal details
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