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PMID: 7021533 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 147 ·No. 2 ·1981-08-00 ·Pages 517-25

Montelone BA, Prakash S, Prakash L

Abstract

The methyl methane sulfonate (MMS)-sensitive mutation mms8-1 increases the rate of spontaneous mitotic intragenic recombination at five heteroallelic loci on three chromosomes. Complementation, segregation, and mapping studies indicate that mms8-1 is allelic to cdc9, known to be defective in deoxyribonucleic acid ligase. Both mms8-1 and cdc9 mutants are lethal in combination with the recombination-defective mutant rad52-1. Genetic analysis of spontaneous red/white sectors in an ade2-1/ade2-1 ade5/+ mms8-1/mms8-1 strain shows nonreciprocal recombinational events involving long chromosome segments. We also observe greater than expected rates of simultaneous recombination at loci on different chromosomes in both wild-type and mms8-1 mutants.

MeSH Terms

Alleles DNA Ligases/genetics Methyl Methanesulfonate/pharmacology Mitosis Mutation Recombination, Genetic Saccharomyces cerevisiae/drug effects,genetics

Chemicals

Methyl Methanesulfonate DNA Ligases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Montelone B A

Prakash S

Prakash L

References (26)

26 references, click to expand

Enhanced mitotic recombination in a ligase-defective mutant of the yeast Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4589-92 PMID: 388447
ALLELIC MAPPING IN YEAST BY X-RAY-INDUCED MITOTIC REVERSION.
Science. 1964 Feb 7;143(3606):581-3 PMID: 14080327
Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion.
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2828-32 PMID: 785473
Coincidence relations between gene conversion and mitotic recombination in Saccharomyces.
Genetics. 1963 Mar;48:321-8 PMID: 13945718
Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants.
Genetics. 1973 Jun;74(2):267-86 PMID: 17248617
The DNA repair capability of cdc9, the Saccharomyces cerevisiae mutant defective in DNA ligase.
Mol Gen Genet. 1979 Feb 16;170(1):89-92 PMID: 375019
Suppressors in yeast.
Curr Top Microbiol Immunol. 1974;64(0):1-47 PMID: 4602646
Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):154-7 PMID: 319455
Induced intragenic recombination in yeast can occur during the G1 mitotic phase.
Nature. 1978 Apr 27;272(5656):795-8 PMID: 347306
Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE.
Genetics. 1980 Jan;94(1):31-50 PMID: 17248995
Evidence that spontaneous mitotic recombination occurs at the two-strand stage.
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436-40 PMID: 360220
Meiotic gene conversion: a signal of the basic recombination event in yeast.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1325-41 PMID: 290446
Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication.
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1195-202 PMID: 4916921
Evidence for joint genic control of spontaneous mutation and genetic recombination during mitosis in Saccharomyces.
Mol Gen Genet. 1977 Jan 18;150(2):127-35 PMID: 320451
A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.
Proc Natl Acad Sci U S A. 1974 May;71(5):2048-51 PMID: 4600786
The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast.
Genetics. 1980 Jan;94(1):51-68 PMID: 17248996
Genetic control of the cell division cycle in yeast.
Science. 1974 Jan 11;183(4120):46-51 PMID: 4587263
Increased spontaneous mitotic segregation in MMS-sensitive mutants of Saccharomyces cerevisiae.
Genetics. 1977 Oct;87(2):229-36 PMID: 200524
Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.
Nature. 1978 Aug 31;274(5674):891-3 PMID: 355897
Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495-502 PMID: 4877274
Genetic analysis of spontaneous half-sectored colonies of Saccharomyces cerevisiae.
Genet Res. 1971 Oct;18(2):179-84 PMID: 4945948
Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12.
J Mol Biol. 1974 May 15;85(2):309-22 PMID: 4600143
Mitotic recombination in yeast: isolation and characterization of mutants with enhanced spontaneous mitotic gene conversion rates.
Genetics. 1980 Apr;94(4):825-39 PMID: 7002715
Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications.
J Bacteriol. 1977 Apr;130(1):167-72 PMID: 323226
The relation of mitotic recombination to DNA replication in yeast pedigrees.
Genetics. 1970 Oct;66(2):291-304 PMID: 5512363
Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae.
Genetics. 1977 May;86(1):33-55 PMID: 195865

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.147.2.517-525.1981

Published

1981-08-00

Pages

517-25

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC216072

Subset

Grants

NIEHS NIH HHS · ES 02433 · United States

NIGMS NIH HHS · GM 19261 · United States

External Links

PubMed source DOI: 10.1128/jb.147.2.517-525.1981 PMC Full Text View journal details

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Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae.

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