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PMID: 7024919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Usage of the three termination codons in a single eukaryotic cell, the Xenopus laevis oocyte.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3835-50

Bienz M, Kubli E, Kohli J, deHenau S, Huez G, Marbaix G, Grosjean H

Abstract

Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogenous mRNAs were then separated on two-dimensional gels. All three termination codons are used in a single cell, the Xenopus laevis oocyte. But a surprisingly low number of readthrough polypeptides were observed from the 600 mRNAs studied in comparison to uninjected oocytes. The experimental data are compared with the conclusions obtained from the compilation of all available termination sequences on eukaryotic and prokaryotic mRNAs. This comparison indicates that the apparent resistance of natural termination codons against readthrough, as observed by the microinjection experiments, cannot be explained by tandem or very close second stop codons. Instead it suggests that specific context sequences around the termination codons may play a role in the efficiency of translation termination.

MeSH Terms
Animals Base Sequence Codon/genetics Female Microinjections Oocytes/metabolism Ovum/metabolism Protein Biosynthesis RNA, Messenger/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics Xenopus
Chemicals
Codon RNA, Messenger RNA, Transfer
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bienz M
Kubli E
Kohli J
deHenau S
Huez G
Marbaix G
Grosjean H
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3835
Published
1981-08-11
Pages
3835-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327395
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.15.3835 PMC Full Text View journal details
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