Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines.

Nucleic acids research ·Vol. 9 ·No. 17 ·1981-09-11 ·Pages 4413-22

Van Charldorp R, Heus HA, Van Knippenberg PH, Joordens J, De Bruin SH, Hilbers CW

Abstract

Fragments comprising the 49 nucleotides from the 3'-end have been purified from 16S ribosomal RNA of wild-type Escherichia coli and from a kasugamycin-resistant mutant that specifically lacks dimethylation of two adjacent adenines near the 3'-terminus. These fragments, obtained after treatment of ribosomes in vitro with the bacteriocin cloacin DF13, were used to study the effect of the methyl groups on the temperature dependent unfolding of double-stranded regions. Both fragments contain at least 3 independent melting transitions, of which the one with the highest Tm corresponds with the unfolding of a nine-basepair long central hairpin. Dimethylation of the adenines in the loop of this hairpin lowers the melting temperature (Tm) by approximately 2 degrees C at 0.2 M NaCl and by about 5 degrees C at 0.15 M NaCl. It is suggested that m6(2)Am6(2)A is more antagonistic to loop formation that ApA and that the function of the methyl groups is to help to destabilize the 3'-terminal hairpin in 16S rRNA in order to facilitate intermolecular interactions.

MeSH Terms

Adenosine/metabolism Chemical Phenomena Chemistry, Physical Escherichia coli/metabolism Methylation Nucleic Acid Conformation RNA, Ribosomal/metabolism Spectrophotometry, Ultraviolet

Chemicals

RNA, Ribosomal Adenosine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Van Charldorp R

Heus H A

Van Knippenberg P H

Joordens J

De Bruin S H

Hilbers C W

References (19)

19 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.17.4413

Published

1981-09-11

Pages

4413-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327444

Subset

External Links

PubMed source DOI: 10.1093/nar/9.17.4413 PMC Full Text View journal details

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Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines.

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