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PMID: 7041126 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 5 ·1982-03-00 ·Pages 1606-10

Stahl S, MacKay P, Magazin M, Bruce SA, Murray K

Abstract

Fragments of hepatitis B virus DNA cloned in plasmid pBR322 carrying the gene for the viral core antigen have been placed under the control of the lac promoter of Escherichia coli. Several of the new recombinants direct higher levels of synthesis of the antigen, but the degree of enhancement varies with the different structures of the plasmids and hence the mRNAs produced. The antigen in crude bacterial lysates is a satisfactory diagnostic reagent for antibodies to the core antigen in serum samples.

MeSH Terms
Antibodies, Viral/analysis DNA, Recombinant Escherichia coli Gene Expression Regulation Hepatitis B Antibodies/analysis Hepatitis B Core Antigens/biosynthesis,genetics Immunoassay Lac Operon Plasmids
Chemicals
Antibodies, Viral DNA, Recombinant Hepatitis B Antibodies Hepatitis B Core Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stahl S
MacKay P
Magazin M
Bruce S A
Murray K
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.5.1606
Published
1982-03-00
Pages
1606-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346024
Subset
IM
Databases
GENBANK
J02204, K01658
External Links
PubMed source DOI: 10.1073/pnas.79.5.1606 PMC Full Text View journal details
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