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PMID: 7061403 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genetic transformation of Rhizobium leguminosarum by plasmid DNA.

Journal of bacteriology ·Vol. 150 ·No. 1 ·1982-04-00 ·Pages 421-4

Bullerjahn GS, Benzinger RH

Abstract

We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.

MeSH Terms
Kanamycin/pharmacology R Factors Rhizobium/drug effects,genetics Tetracycline/pharmacology Transformation, Bacterial
Chemicals
Kanamycin Tetracycline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bullerjahn G S
Benzinger R H
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.150.1.421-424.1982
Published
1982-04-00
Pages
421-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220134
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.150.1.421-424.1982 PMC Full Text View journal details
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