A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.

The Journal of experimental medicine ·Vol. 156 ·No. 6 ·1982-12-01 ·Pages 1848-53

Kemp DJ, Adams JM, Mottram PL, Thomas WR, Walker ID, Miller JF

Abstract

Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in two B cell lymphomas and in splenic B cells. Less than one molecule per cell of RNA, exactly complementary to the DNA probes used, would have been detected in these T cell populations. The results thus seriously question the proposition that T cells use the B cell VH repertoire to code for antigen receptors.

MeSH Terms

Animals Base Sequence Binding Sites, Antibody/genetics Hybridomas/analysis Immunoglobulin Variable Region/genetics Mice Mice, Inbred A Mice, Inbred BALB C RNA, Messenger/genetics,immunology Receptors, Antigen, B-Cell/genetics Receptors, Antigen, T-Cell/genetics

Chemicals

Immunoglobulin Variable Region RNA, Messenger Receptors, Antigen, B-Cell Receptors, Antigen, T-Cell

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kemp D J

Adams J M

Mottram P L

Thomas W R

Walker I D

Miller J F

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24 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.156.6.1848

Published

1982-12-01

Pages

1848-53

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2186877

Subset

External Links

PubMed source DOI: 10.1084/jem.156.6.1848 PMC Full Text View journal details

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A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.

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