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PMID: 7298581 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Improved generalized transducing bacteriophage for Caulobacter crescentus.

Journal of bacteriology ·Vol. 148 ·No. 2 ·1981-11-00 ·Pages 734-5

Bender RA

Abstract

Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and eliminated the requirement for irradiation of transducing lysates to prevent killing of transductants on the plate.

MeSH Terms
Bacteria/genetics Bacteriophages/genetics,isolation & purification Mutation Transduction, Genetic
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bender R A
References (8)
8 references, click to expand
  1. Genetic studies on bacteriophage P1.
    Virology. 1968 Dec;36(4):564-74 PMID: 4881411
  2. The caulobacters: ubiquitous unusual bacteria.
    Microbiol Rev. 1981 Mar;45(1):123-79 PMID: 7012570
  3. Ammonia assimilation and glutamate formation in Caulobacter crescentus.
    J Bacteriol. 1978 Jan;133(1):225-30 PMID: 22537
  4. Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus.
    J Bacteriol. 1979 Jan;137(1):627-34 PMID: 762024
  5. Indirect ultraviolet-reactivation of phage lambda.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):144-7 PMID: 4589889
  6. Differentiation in the Caulobacter cell cycle.
    Annu Rev Microbiol. 1976;30:377-407 PMID: 185940
  7. Caulobacter cresentus mutant defective in membrane phospholipid synthesis.
    J Bacteriol. 1979 Nov;140(2):612-9 PMID: 500564
  8. Generalized Transduction in CAULOBACTER CRESCENTUS.
    Genetics. 1977 Nov;87(3):391-9 PMID: 17248770
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.2.734-735.1981
Published
1981-11-00
Pages
734-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216264
Subset
IM
Grants
NIAID NIH HHS · AI 15822 · United States
NIGMS NIH HHS · GM 27111 · United States
External Links
PubMed source DOI: 10.1128/jb.148.2.734-735.1981 PMC Full Text View journal details
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