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PMID: 7312623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamide-gels.

Nucleic acids research ·Vol. 9 ·No. 19 ·1981-10-10 ·Pages 4967-79

Frank R, Müller D, Wolff C

Abstract

Nature and stability of secondary structures formed from DNA-fragments during electrophoresis in urea containing polyacrylamide-gels were investigated. Duplices and especially hairpin loops were found to be surprisingly stable. Polyacrylamide-gels containing high concentrations of formamide effectively melt these structures. Such gels will improve nucleotide sequence analysis in regions of dyad symmetry that often cause 'band compressions' under standard electrophoresis conditions.

MeSH Terms
Electrophoresis, Polyacrylamide Gel/methods Formamides Nucleic Acid Conformation Nucleic Acid Denaturation Oligodeoxyribonucleotides/analysis Oligonucleotides/analysis Urea
Chemicals
Formamides Oligodeoxyribonucleotides Oligonucleotides Urea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frank R
Müller D
Wolff C
References (9)
9 references, click to expand
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  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
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    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  8. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  9. DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.
    Nucleic Acids Res. 1979;6(6):2069-87 PMID: 461182
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.19.4967
Published
1981-10-10
Pages
4967-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327492
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.19.4967 PMC Full Text View journal details
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