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PMID: 7335492 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.

Nucleic acids research ·Vol. 9 ·No. 24 ·1981-12-21 ·Pages 6709-22

Di Segni G, Carrara G, Tocchini-Valentini GR, Shoulders CC, Baralle FE

Abstract

The sequence of 2965 nucleotides 5' of the human epsilon-globin gene has been completed. It includes two Alu family repeats present in an inverted configuration. Only the one located farthest from the gene was active as template for RNA polymerase III in a transcription system prepared from nuclei of Xenopus laevis oocytes. This selective transcription may be explained by the lack of homology of the first 45 nucleotides of the non transcribed repeat with other members of the Alu family. In fact this region includes one of the homology blocks described for other RNA polymerase III templates.

MeSH Terms

Animals Base Sequence DNA, Recombinant Female Genes Globins/genetics Humans In Vitro Techniques Oocytes/metabolism Repetitive Sequences, Nucleic Acid Transcription, Genetic Xenopus laevis

Chemicals

DNA, Recombinant Globins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Di Segni G

Carrara G

Tocchini-Valentini G R

Shoulders C C

Baralle F E

References (20)

20 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.24.6709

Published

1981-12-21

Pages

6709-22

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327635

Subset

Databases

GENBANK

J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067

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PubMed source DOI: 10.1093/nar/9.24.6709 PMC Full Text View journal details

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Selective in vitro transcription of one of the two Alu family repeats present in the 5' flanking region of the human epsilon-globin gene.

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