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PMID: 7373718 Published · ppublish English Journal Article

Reovirus progeny subviral particles synthesize uncapped mRNA.

Journal of virology ·Vol. 34 ·No. 2 ·1980-05-00 ·Pages 497-505

Zarbl H, Skup D, Millward S

Abstract

Reovirus progeny subviral particles were isolated from L-cells at late times postinfection. It has been shown (D. Skup and S. Millward, J. Virol. 34: 490--496, 1980) that these progeny subviral particles have masked capping enzymes, indicating that mRNA synthesized by these particles should be uncapped. When progeny subviral particles were used for mRNA synthesis in vitro, they failed to incorporate the beta-phosphate of [beta-32P]GTP into the 5' terminal. Direct analysis of reovirus mRNA synthesized by progeny subviral particles in the presence of either [alpha-32P]GTP or [alpha-32P]CTP indicated that the 5' terminal was uncapped, having the structure pGpC... The implications of this finding to the reovirus replicative cycle are discussed.

MeSH Terms
Chymotrypsin/pharmacology Mammalian orthoreovirus 3/growth & development,metabolism Protein Biosynthesis RNA Caps/metabolism RNA, Messenger/analysis,biosynthesis,genetics RNA, Viral/analysis,biosynthesis,genetics Reoviridae/metabolism Virion/metabolism
Chemicals
RNA Caps RNA, Messenger RNA, Viral Chymotrypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zarbl H
Skup D
Millward S
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.2.497-505.1980
Published
1980-05-00
Pages
497-505
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288729
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.34.2.497-505.1980 PMC Full Text View journal details
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