Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii.

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 269-75

Malnoë P, Rochaix JD

Abstract

The genes coding for 4S RNA have been localized on the physical map of the chloroplast genome of Chlamydomonas reinhardii by hybridizing 32P-labelled 4S RNA to EcoRI, BamHI, Bg1II and Hind III chloroplast DNA digests and to hybrid plasmids containing EcoRI and Bam HI chloroplast DNA fragments. At least 10 EcoRI and 7 Bam HI fragments carry sequences coding for 4S RNA. These genes are interspersed throughout the genome. The spacer between the 16S and 23S ribosomal RNA genes, which is repeated twice per chloroplast DNA molecule, codes for at least one 4S RNA, shown to be transcribed from the same strand as the ribosomal RNAs.

MeSH Terms

Chlamydomonas/genetics Chloroplasts DNA/genetics Genes Genetic Linkage Nucleic Acid Hybridization RNA/genetics RNA, Ribosomal/genetics

Chemicals

RNA, Ribosomal RNA DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Malnoë P

Rochaix J D

References (13)

13 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267618

Published

1978-11-09

Pages

269-75

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267618

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Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii.

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