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PMID: 74421 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A replication-defective variant of Moloney murine leukemia virus. I. Biological characterization.

Journal of virology ·Vol. 25 ·No. 1 ·1978-01-00 ·Pages 146-56

Rein AL, Gerwin BI, Bassin RH, Schwarm L, Schidlovsky G

Abstract

We have studied the virus produced by a clone, termed 8A, that was isolated from a culture of murine sarcoma virus-transformed mouse cells after superinfection with Moloney murine leukemia virus (MuLV-M). Clone 8A produced high levels of type C virus particles, but only a low titer of infectious murine sarcoma virus and almost no infectious MuLV. When fresh cultures of mouse cells were infected with undiluted clone 8A culture fluids, they released no detectable pogeny virus for several weeks after infection. Fully infectious MuLV was then produced in these cultures. This virus was indistinguishable from MuLV-M by nucleic acid hybridization tests and in its insensitivity to Fv-1 restriction. It also induced thymic lymphomas in BALB/c mice. To explain these results, we propose that cone 8A is infected with a replication-defective variant of MuLV-M. Particles produced by clone 8A, containing this defective genome, can establish an infection in fresh cells but cannot produce progency virus at detectable levels. Several weeks after infection, the defect in the viral genome is corrected by back-mutation or by recombination with endogenous viral genomes, resulting in the formation of fully infectious progeny MuLV. The progeny MuLV'S that arose in two different experiments were found to be genetically different from each other. This is consistent with the hypothesis that, in each experiment, the progeny virus is formed clone 8A cells and assayed for infectivity by the calcium phosphate transfection technique. No detectable MuLV was produced by cells treated with this DNA. This finding, along with positive results obtained in control experiments, indicates that clone 8A cells do not contain a normal MuLV provirus.

MeSH Terms
Cell Line Genetic Variation Moloney murine leukemia virus/enzymology,genetics,growth & development RNA-Directed DNA Polymerase/metabolism Transfection Virus Replication
Chemicals
RNA-Directed DNA Polymerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rein A L
Gerwin B I
Bassin R H
Schwarm L
Schidlovsky G
References (53)
53 references, click to expand
  1. A novel murine oncornavirus with dual eco- and xenotropic properties.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5150-5 PMID: 1061100
  2. Recombination between endogenous and exogenous RNA tumor virus genes as analyzed by nucleic acid hybridization.
    J Virol. 1975 Jun;15(6):1367-77 PMID: 167190
  3. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses.
    Virology. 1975 May;65(1):128-34 PMID: 167514
  4. Murine sarcoma virus pseudotypes acquire a determinant specifying N or B tropism from leukaemia virus during rescue.
    Nature. 1975 Jul 17;256(5514):223-5 PMID: 168499
  5. High spontaneous mutation rate of an avian sarcoma virus.
    J Virol. 1975 Jan;17(1):74-84 PMID: 173893
  6. Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction.
    Cell. 1976 Mar;7(3):373-9 PMID: 181144
  7. Amphotropic host range of naturally occuring wild mouse leukemia viruses.
    J Virol. 1976 Jul;19(1):13-8 PMID: 181592
  8. Clonal heterogeneity of wild mouse leukemia viruses: host ranges and antigenicity.
    Virology. 1976 Sep;73(2):532-6 PMID: 183368
  9. Six-NB-tropic murine leukemia viruses derived from a B-tropic virus of BALB/c have altered p30.
    J Virol. 1977 Jan;21(1):309-18 PMID: 189069
  10. A new class of murine leukemia virus associated with development of spontaneous lymphomas.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):789-92 PMID: 191826
  11. Friend strain of spleen focus-forming virus: a recombinant between mouse type C ecotropic viral sequences and sequences related to xenotropic virus.
    J Virol. 1977 May;22(2):361-72 PMID: 194058
  12. Donation of N- or B-tropic phenotype to NB-tropic murine leukemia virus during mixed infections.
    J Virol. 1977 Jun;22(3):626-33 PMID: 195081
  13. Evidence for recombination between N- and B-tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Virol. 1977 Sep;23(3):700 PMID: 197267
  14. Plaque assay techniques for murine leukemia viruses.
    Virology. 1970 Dec;42(4):1136-9 PMID: 4099080
  15. Isolation of temperature-sensitive mutants of murine sarcoma virus.
    J Virol. 1972 Oct;10(4):653-7 PMID: 4117345
  16. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA.
    Virology. 1973 Feb;51(2):370-82 PMID: 4120907
  17. Characterization of temperature-sensitive mutants of murine leukemia virus.
    Virology. 1973 Jul;54(1):53-9 PMID: 4123462
  18. A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):602-6 PMID: 4132531
  19. Revertants of mouse cells transformed by murine sarcoma virus. 3. Metastable expression of virus functions in revertants retransformed by murine sarcoma virus.
    Virology. 1974 May;59(1):217-29 PMID: 4133134
  20. Systematics of RNA tumor viruses and virus-like particles of human origin.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3177-81 PMID: 4137960
  21. Tissue culture assay of helper activity of murine leukemia virus for murine sarcoma virus.
    J Natl Cancer Inst. 1968 Jun;40(6):1199-212 PMID: 4298946
  22. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
    J Cell Physiol. 1968 Oct;72(2):141-8 PMID: 4301006
  23. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
    J Virol. 1969 Nov;4(5):549-53 PMID: 4311790
  24. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures.
    J Virol. 1970 Feb;5(2):221-5 PMID: 4317349
  25. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures.
    Int J Cancer. 1970 Jul 15;6(1):95-107 PMID: 4319233
  26. Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520-4 PMID: 4327005
  27. Detection of some murine leukemia virus antigens in virus particles derived from 3T3 cells transformed only by murine sarcoma virus.
    Virology. 1972 Jan;47(1):229-35 PMID: 4333569
  28. Comparison of murine sarcoma viruses in nonproducer and S + L - -transformed cells.
    J Virol. 1972 Apr;9(4):701-4 PMID: 4336564
  29. Isolation of temperature-sensitive mutants of murine leukemia virus.
    Virology. 1972 Jun;48(3):749-56 PMID: 4337991
  30. Virus recovery in chicken cells tested with Rous sarcoma cell DNA.
    Nat New Biol. 1972 May 10;237(71):35-9 PMID: 4338041
  31. A genetic locus for inducibility of C-type in BALB-c cells: the effect of a nonlinked regulatory gene on detection of virus after chemical activation.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2798-801 PMID: 4344375
  32. Genetic recombinants and heterozygotes derived from endogenous and exogenous avian RNA tumor viruses.
    Virology. 1973 Apr;52(2):535-52 PMID: 4350228
  33. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
    Cancer Res. 1973 Dec;33(12):3231-8 PMID: 4357355
  34. Temperature-sensitive mutants of murine leukemia virus. 3. Mutants defective in helper functions for sarcoma virus fixation.
    Virology. 1974 Mar;58(1):294-7 PMID: 4362551
  35. Nucleic acid homology of murine type-C viral genes.
    J Virol. 1974 Dec;14(6):1394-403 PMID: 4372403
  36. Evidence for genetic recombination between endogenous and exogenous mouse RNA type C viruses.
    Cell. 1974 Jun;2(2):87-94 PMID: 4376054
  37. Dual susceptibility of A 3T3 mouse cell line to infection by N- and B-tropic murine leukemia virus: apparent lack of expression of the FV-1 gene.
    Int J Cancer. 1974 Jul 15;14(1):106-13 PMID: 4376504
  38. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  39. Rapid, selective procedure for isolation of spontaneous temperature-sensitive mutants of Moloney leukemia virus.
    Virology. 1973 Feb;51(2):424-31 PMID: 4735036
  40. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  41. Rapid cell culture assay technic for murine leukaemia viruses.
    Nature. 1971 Feb 19;229(5286):564-6 PMID: 4925356
  42. Quantitative nucleotide sequence relationships of mammalian RNA tumor viruses.
    J Virol. 1975 Jun;15(6):1396-408 PMID: 49442
  43. Variants of N-tropic leukemia virus derived from BALB/c mice.
    J Virol. 1975 Oct;16(4):991-9 PMID: 51938
  44. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):780-6 PMID: 5219685
  45. Thermolabile reverse transcriptase of a mammalian leukemia virus mutant temperature sensitive in its replication and sarcoma virus helper functions.
    J Virol. 1975 Dec;16(6):1476-82 PMID: 53294
  46. Murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine S + L-cells.
    Virology. 1976 Apr;70(2):313-23 PMID: 57666
  47. Isolation and identification of a helper virus found in the Moloney sarcoma-leukemia virus complex.
    J Natl Cancer Inst. 1969 Apr;42(4):605-22 PMID: 5775808
  48. The isolation and replica plating of mammalian cell clones.
    Exp Cell Res. 1969 Feb;54(2):271-5 PMID: 5776484
  49. Endogenous ecotropic mouse type C viruses deficient in replication and production of XC plaques.
    J Virol. 1976 May;18(2):411-7 PMID: 58071
  50. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus.
    J Bacteriol. 1966 Oct;92(4):1133-40 PMID: 5926742
  51. Effect of Fv-1 gene product on synthesis of N-tropic and B-tropic murine leukemia viral RNA.
    Cell. 1976 Jan;7(1):33-9 PMID: 59632
  52. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class.
    J Virol. 1976 Jul;19(1):19-25 PMID: 59816
  53. Interactions of murine leukemia virus core components: characterization of reverse transcriptase packaged in the absence of 70S genomic RNA.
    J Virol. 1977 Nov;24(2):478-88 PMID: 72160
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.25.1.146-156.1978
Published
1978-01-00
Pages
146-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353911
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.25.1.146-156.1978 PMC Full Text View journal details
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