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PMID: 7505291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

Journal of clinical microbiology ·Vol. 31 ·No. 11 ·1993-11-00 ·Pages 2882-9

Kirschner P, Springer B, Vogel U, Meier A, Wrede A, Kiekenbeck M, Bange FC, Böttger EC

Abstract

Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.

MeSH Terms
Base Sequence Genotype Humans Molecular Sequence Data Mycobacterium/genetics Mycobacterium Infections/diagnosis Polymerase Chain Reaction RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kirschner P
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
Springer B
Vogel U
Meier A
Wrede A
Kiekenbeck M
Bange F C
Böttger E C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.11.2882-2889.1993
Published
1993-11-00
Pages
2882-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC266149
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.31.11.2882-2889.1993 PMC Full Text View journal details
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