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PMID: 7512260 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.

Nucleic acids research ·Vol. 22 ·No. 6 ·1994-03-25 ·Pages 1059-67

Matunis MJ, Xing J, Dreyfuss G

Abstract

More than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) are associated with pre-mRNAs in the nucleus of mammalian cells and these proteins appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. The arrangement of hnRNP proteins on pre-mRNAs is likely to be unique for each RNA and may be determined by the different RNA-binding preferences of each of these proteins. hnRNP F (M(r) = 53 kD, pI = 6.1) and hnRNP H (M(r) = 56 kD, pI = 6.7-7.1) are abundant components of immunopurified hnRNP complexes and they have distinct nucleic acid binding properties. Unlike other hnRNP proteins which display a varying range of affinities for different ribonucleotidehomopolymers and ssDNA, hnRNP F and hnRNP H bind only to poly(rG) in vitro. hnRNP F and hnRNP H were purified from HeLa cells by poly(rG) affinity chromatography and oligonucleotides derived from peptide sequences were used to isolate a cDNA encoding hnRNP F. The predicted amino acid sequence of hnRNP F revealed a novel protein with three repeated domains related to the RNP consensus sequence RNA-binding domain. Monoclonal antibodies produced against bacterially expressed hnRNP F were specific for both hnRNP F and hnRNP H and recognized related proteins in divergent organisms, including in the yeast Saccharomyces cerevisiae. hnRNP F and hnRNP H are thus highly related immunologically and they share identical peptides. Interestingly, immunofluorescence microscopy revealed that hnRNP F and hnRNP H are concentrated in discrete regions of the nucleoplasm, in contrast to the general nucleoplasmic distribution of previously characterized hnRNP proteins. The unique RNA-binding properties, amino acid sequence and distinct intranuclear localization of hnRNP F and hnRNP H make them novel hnRNP proteins that are likely to be important for the processing of RNAs containing guanosine-rich sequences.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cattle Cell Line Cell Nucleus/chemistry Chromatography, Affinity Consensus Sequence Conserved Sequence DNA, Complementary/chemistry,isolation & purification HeLa Cells Heterogeneous-Nuclear Ribonucleoprotein Group F-H Heterogeneous-Nuclear Ribonucleoproteins Humans Kidney Molecular Sequence Data Poly G/metabolism RNA/metabolism RNA Precursors/metabolism RNA, Messenger/metabolism Ribonucleoproteins/chemistry,genetics,isolation & purification Subcellular Fractions/chemistry
Chemicals
DNA, Complementary Heterogeneous-Nuclear Ribonucleoprotein Group F-H Heterogeneous-Nuclear Ribonucleoproteins RNA Precursors RNA, Messenger Ribonucleoproteins Poly G RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matunis M J
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148.
Xing J
Dreyfuss G
References (57)
57 references, click to expand
  1. Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
    Mol Cell Biol. 1988 May;8(5):2237-41 PMID: 3386636
  2. RNA annealing activities in HeLa nuclei.
    EMBO J. 1994 Jan 1;13(1):213-21 PMID: 7508381
  3. Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.
    Mol Cell Biol. 1987 May;7(5):1731-9 PMID: 3110598
  4. Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
    EMBO J. 1989 Dec 20;8(13):4163-70 PMID: 2531658
  5. hnRNP I, the polypyrimidine tract-binding protein: distinct nuclear localization and association with hnRNAs.
    Nucleic Acids Res. 1992 Jul 25;20(14):3671-8 PMID: 1641332
  6. The role of exon sequences in splice site selection.
    Genes Dev. 1993 Mar;7(3):407-18 PMID: 8449402
  7. In vitro reconstitution of 35S ribonucleoprotein complexes.
    Biochemistry. 1983 Sep 13;22(19):4592-600 PMID: 6626517
  8. Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):6970-4 PMID: 3313383
  9. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies.
    Mol Cell Biol. 1984 Jun;4(6):1104-14 PMID: 6204191
  10. Characterization of cDNAs encoding the polypyrimidine tract-binding protein.
    Genes Dev. 1991 Jul;5(7):1224-36 PMID: 1906035
  11. Association of individual hnRNP proteins and snRNPs with nascent transcripts.
    J Cell Biol. 1993 Apr;121(2):219-28 PMID: 8468343
  12. Phenylalanines that are conserved among several RNA-binding proteins form part of a nucleic acid-binding pocket in the A1 heterogeneous nuclear ribonucleoprotein.
    J Biol Chem. 1988 Mar 5;263(7):3307-13 PMID: 2830282
  13. Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A.
    Nature. 1990 Dec 6;348(6301):515-20 PMID: 2147232
  14. RNA binding specificity of hnRNP proteins: a subset bind to the 3' end of introns.
    EMBO J. 1988 Nov;7(11):3519-29 PMID: 3208740
  15. Nuclear organization of pre-mRNA processing.
    Curr Opin Cell Biol. 1993 Jun;5(3):442-7 PMID: 8352961
  16. Quantitative determination that one of two potential RNA-binding domains of the A protein component of the U1 small nuclear ribonucleoprotein complex binds with high affinity to stem-loop II of U1 RNA.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6393-7 PMID: 1696729
  17. Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.
    Nature. 1990 Jun 7;345(6275):502-6 PMID: 2140872
  18. Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro.
    J Biol Chem. 1993 Aug 25;268(24):18249-58 PMID: 8349701
  19. RNA recognition: towards identifying determinants of specificity.
    Trends Biochem Sci. 1991 Jun;16(6):214-20 PMID: 1716386
  20. An RNA-binding protein specifically interacts with a functionally important domain of the downstream element of the simian virus 40 late polyadenylation signal.
    Mol Cell Biol. 1991 Oct;11(10):5312-20 PMID: 1656229
  21. Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box.
    EMBO J. 1992 Jul;11(7):2655-64 PMID: 1628625
  22. RNA ligands: out of shape but fir for recognition.
    Curr Biol. 1993 Jun 1;3(6):375-7 PMID: 15335736
  23. Structure and function of telomeres.
    Nature. 1991 Apr 18;350(6319):569-73 PMID: 1708110
  24. Interaction of the RNA-binding domain of the hnRNP C proteins with RNA.
    EMBO J. 1992 Sep;11(9):3289-95 PMID: 1380452
  25. Characterization and molecular cloning of polypyrimidine tract-binding protein: a component of a complex necessary for pre-mRNA splicing.
    Genes Dev. 1991 Jul;5(7):1237-51 PMID: 1906036
  26. Immunological methods for purification and characterization of heterogeneous nuclear ribonucleoprotein particles.
    Methods Enzymol. 1990;181:317-25 PMID: 2143256
  27. Cell cycle-regulated phosphorylation of the pre-mRNA-binding (heterogeneous nuclear ribonucleoprotein) C proteins.
    Mol Cell Biol. 1993 Sep;13(9):5762-70 PMID: 8395012
  28. G+C-rich tract in 5' end of human introns.
    J Mol Biol. 1992 Sep 5;227(1):108-13 PMID: 1522582
  29. Heterogeneous nuclear ribonucleoprotein particles and the pathway of mRNA formation.
    Trends Biochem Sci. 1988 Mar;13(3):86-91 PMID: 3072706
  30. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  31. Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
    Mol Cell Biol. 1993 Jul;13(7):4301-10 PMID: 8321232
  32. Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.
    Mol Cell Biol. 1992 Jan;12(1):164-71 PMID: 1729596
  33. Heterogeneous nuclear ribonucleoproteins: role in RNA splicing.
    Science. 1986 Mar 28;231(4745):1534-9 PMID: 3952495
  34. Properties of BGP1, a poly(dG)-binding protein from chicken erythrocytes.
    Nucleic Acids Res. 1990 Sep 11;18(17):5119-26 PMID: 2402439
  35. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  36. Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9788-92 PMID: 2557628
  37. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  38. Heterogeneous nuclear ribonucleoprotein A1 catalyzes RNA.RNA annealing.
    Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):895-9 PMID: 1371011
  39. Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.
    Nucleic Acids Res. 1991 Oct 11;19(19):5237-45 PMID: 1681508
  40. A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2575-87 PMID: 2687284
  41. X-ray fiber diffraction and model-building study of polyguanylic acid and polyinosinic acid.
    J Mol Biol. 1975 Feb 25;92(2):181-92 PMID: 1142423
  42. The coiled body.
    Trends Cell Biol. 1993 Jun;3(6):198-204 PMID: 14731494
  43. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2.
    Cell. 1992 Jan 24;68(2):365-75 PMID: 1531115
  44. Monoclonal antibody characterization of the C proteins of heterogeneous nuclear ribonucleoprotein complexes in vertebrate cells.
    J Cell Biol. 1984 Dec;99(6):1997-204 PMID: 6209285
  45. The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
    Nucleic Acids Res. 1993 Feb 11;21(3):439-46 PMID: 8441656
  46. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing.
    Annu Rev Cell Biol. 1991;7:559-99 PMID: 1839712
  47. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
    Nature. 1992 Feb 20;355(6362):730-2 PMID: 1371331
  48. hnRNP proteins and the biogenesis of mRNA.
    Annu Rev Biochem. 1993;62:289-321 PMID: 8352591
  49. Isolation of the heterogeneous nuclear RNA-ribonucleoprotein complex (hnRNP): a unique supramolecular assembly.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7471-5 PMID: 6594697
  50. Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins.
    Genes Dev. 1988 Feb;2(2):215-27 PMID: 3129338
  51. Studies of the strand-annealing activity of mammalian hnRNP complex protein A1.
    Biochemistry. 1990 Dec 4;29(48):10717-22 PMID: 1703006
  52. RNA-binding proteins as developmental regulators.
    Genes Dev. 1989 Apr;3(4):431-7 PMID: 2470643
  53. RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2495-9 PMID: 1826055
  54. 1H, 13C, and 15N NMR assignments and global folding pattern of the RNA-binding domain of the human hnRNP C proteins.
    Biochemistry. 1992 Jul 14;31(27):6254-65 PMID: 1385725
  55. Ribonucleoproteins package 700 nucleotides of pre-mRNA into a repeating array of regular particles.
    Mol Cell Biol. 1988 Jul;8(7):2884-95 PMID: 3405221
  56. Associations between distinct pre-mRNA splicing components and the cell nucleus.
    EMBO J. 1991 Nov;10(11):3467-81 PMID: 1833187
  57. Tetramerization of an RNA oligonucleotide containing a GGGG sequence.
    Nature. 1991 May 23;351(6324):331-2 PMID: 1709723
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.6.1059
Published
1994-03-25
Pages
1059-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307930
Subset
IM
Databases
GENBANK
L28010
External Links
PubMed source DOI: 10.1093/nar/22.6.1059 PMC Full Text View journal details
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