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PMID: 7515148 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Preferential deadenylation of Hsp70 mRNA plays a key role in regulating Hsp70 expression in Drosophila melanogaster.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 3646-59

Dellavalle RP, Petersen R, Lindquist S

Abstract

Following a standard heat shock, approximately 40% of Hsp70 transcripts in Drosophila melanogaster lack a poly(A) tail. Since heat shock disrupts other aspects of RNA processing, this observation suggested that heat might disrupt polyadenylation as well. We find, however, that as the temperature is increased a larger fraction of Hsp70 RNA is polyadenylated. Poly(A)-deficient Hsp70 RNAs arise not from a failure in polyadenylation but from the rapid and selective removal of poly(A) from previously adenylated transcripts. Poly(A) removal is highly regulated: poly(A) is (i) removed much more rapidly from Hsp70 RNAs than from Hsp23 RNAs, (ii) removed more rapidly after mild heat shocks than after severe heat shocks, and (iii) removed more rapidly after a severe heat shock if cells have first been conditioned by a mild heat treatment. Poly(A) seems to be removed by simple deadenylation rather than by endonucleolytic cleavage 5' of the adenylation site. During recovery from heat shock, deadenylation is rapidly followed by degradation. In cells maintained at high temperatures, however, the two processes are uncoupled and Hsp70 RNAs are deadenylated without being degraded. These deadenylated mRNAs are translated with low efficiency. Deadenylation therefore allows Hsp70 synthesis to be repressed even when degradation of the mRNA is blocked. Poly(A) tail shortening appears to play a key role in regulating Hsp70 expression.

Related Genes
Hsp23 Hsp70 Hsp83
MeSH Terms
Animals Antisense Elements (Genetics) Base Sequence Cell Line Drosophila melanogaster/genetics,metabolism Gene Expression Regulation Heat-Shock Proteins/biosynthesis,genetics Hot Temperature Molecular Sequence Data Oligonucleotide Probes Poly A/isolation & purification,metabolism RNA/isolation & purification,metabolism RNA, Messenger/isolation & purification,metabolism Ribonuclease H
Chemicals
Antisense Elements (Genetics) Heat-Shock Proteins Oligonucleotide Probes RNA, Messenger Poly A RNA Ribonuclease H
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dellavalle R P
Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, Illinois 60637.
Petersen R
Lindquist S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.3646-3659.1994
Published
1994-06-00
Pages
3646-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358732
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GM 07281 · United States
NIGMS NIH HHS · GM 25874 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.6.3646-3659.1994 PMC Full Text View journal details
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