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PMID: 7536299 Published · ppublish English Journal Article Review

All you wanted to know about SELEX.

Molecular biology reports ·Vol. 20 ·No. 2 ·1994-00-00 ·Pages 97-107

Klug SJ, Famulok M

Abstract

In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

MeSH Terms
Base Sequence Biological Evolution Chromatography, Affinity DNA/chemistry,genetics,isolation & purification DNA Primers DNA-Binding Proteins Ligands Molecular Biology/methods Molecular Sequence Data Molecular Structure RNA/chemistry,genetics,isolation & purification RNA, Catalytic/isolation & purification RNA-Binding Proteins
Chemicals
DNA Primers DNA-Binding Proteins Ligands RNA, Catalytic RNA-Binding Proteins RNA DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klug S J
Institut für Molekulare Biochemie, Genzentrum der Ludwig-Maximillian-Universität Müchen, Germany.
Famulok M
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00996358
Published
1994-00-00
Pages
97-107
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00996358 View journal details
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