Home LiteratureArticle Details
PMID: 7543593 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Modification of retroviral RNA by double-stranded RNA adenosine deaminase.

Journal of virology ·Vol. 69 ·No. 9 ·1995-09-00 ·Pages 5878-82

Hajjar AM, Linial ML

Abstract

In this report, we describe a recombinant provirus generated during in vitro passage that contains a short region of adenosine-to-guanosine hypermutation. The hypermutated region is restricted to complementary sequences present in the recombinant provirus. We propose that a duplex was formed in the recombinant RNA prior to reverse transcription. This duplex was a substrate for double-stranded RNA adenosine deaminase, an activity found in all cells examined that deaminates A in double-stranded RNA, converting it to inosine, which is further converted to a guanosine by reverse transcription. It appears that cis viral sequences facilitated the A-->G transitions.

Related Genes
env pol
MeSH Terms
Adenosine Adenosine Deaminase/metabolism Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Line DNA Primers Genes, env Genes, pol Guanosine Molecular Sequence Data Mutation Polymerase Chain Reaction Proviruses/genetics Quail RNA/biosynthesis,metabolism RNA, Antisense RNA, Viral/biosynthesis,metabolism RNA-Binding Proteins Repetitive Sequences, Nucleic Acid Substrate Specificity
Chemicals
DNA Primers RNA, Antisense RNA, Viral RNA, recombinant RNA-Binding Proteins Guanosine RNA ADARB1 protein, human Adenosine Deaminase Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hajjar A M
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Linial M L
References (37)
37 references, click to expand
  1. Functional and biological properties of an avian variant long terminal repeat containing multiple A to G conversions in the U3 sequence.
    J Virol. 1994 Aug;68(8):4759-67 PMID: 8035477
  2. Molecular cloning of cDNA for double-stranded RNA adenosine deaminase, a candidate enzyme for nuclear RNA editing.
    Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11457-61 PMID: 7972084
  3. Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase.
    EMBO J. 1994 Dec 1;13(23):5701-11 PMID: 7527340
  4. Hypermutagenesis of RNA using human immunodeficiency virus type 1 reverse transcriptase and biased dNTP concentrations.
    Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11787-91 PMID: 7527543
  5. Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase.
    Mol Cell Biol. 1995 Mar;15(3):1389-97 PMID: 7862132
  6. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail.
    Cell. 1977 May;11(1):95-103 PMID: 194709
  7. Vesicular stomatitis virus defective interfering particles can contain extensive genomic sequence rearrangements and base substitutions.
    Cell. 1984 Apr;36(4):915-24 PMID: 6323026
  8. Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
    Cell. 1987 Feb 27;48(4):599-605 PMID: 2434240
  9. A developmentally regulated activity that unwinds RNA duplexes.
    Cell. 1987 Feb 27;48(4):607-13 PMID: 2434241
  10. Creation of a processed pseudogene by retroviral infection.
    Cell. 1987 Apr 10;49(1):93-102 PMID: 3030568
  11. Biased hypermutation and other genetic changes in defective measles viruses in human brain infections.
    Cell. 1988 Oct 21;55(2):255-65 PMID: 3167982
  12. An unwinding activity that covalently modifies its double-stranded RNA substrate.
    Cell. 1988 Dec 23;55(6):1089-98 PMID: 3203381
  13. Biased hypermutation of viral RNA genomes could be due to unwinding/modification of double-stranded RNA.
    Cell. 1989 Feb 10;56(3):331 PMID: 2914324
  14. A double-stranded RNA unwinding activity introduces structural alterations by means of adenosine to inosine conversions in mammalian cells and Xenopus eggs.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2647-51 PMID: 2704740
  15. Specificity and mechanism of error-prone replication by human immunodeficiency virus-1 reverse transcriptase.
    J Biol Chem. 1989 Oct 5;264(28):16948-56 PMID: 2476448
  16. Unusual features of integrated cDNAs generated by infection with genome-free retroviruses.
    Mol Cell Biol. 1990 May;10(5):1891-900 PMID: 2325641
  17. Retroviral RNA packaging: sequence requirements and implications.
    Curr Top Microbiol Immunol. 1990;157:125-52 PMID: 2394131
  18. Double-stranded RNA unwinding and modifying activity is detected ubiquitously in primary tissues and cell lines.
    Mol Cell Biol. 1990 Oct;10(10):5586-90 PMID: 2398905
  19. Selection, recombination, and G----A hypermutation of human immunodeficiency virus type 1 genomes.
    J Virol. 1991 Apr;65(4):1779-88 PMID: 2002543
  20. Role of biased hypermutation in evolution of subacute sclerosing panencephalitis virus from progenitor acute measles virus.
    J Virol. 1991 May;65(5):2191-9 PMID: 2016756
  21. Tat-dependent adenosine-to-inosine modification of wild-type transactivation response RNA.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8096-100 PMID: 1896456
  22. RNA editing in brain controls a determinant of ion flow in glutamate-gated channels.
    Cell. 1991 Oct 4;67(1):11-9 PMID: 1717158
  23. Substrate specificity of the dsRNA unwinding/modifying activity.
    EMBO J. 1991 Nov;10(11):3523-32 PMID: 1915306
  24. The mechanism of adenosine to inosine conversion by the double-stranded RNA unwinding/modifying activity: a high-performance liquid chromatography-mass spectrometry analysis.
    Biochemistry. 1991 Dec 10;30(49):11507-14 PMID: 1747369
  25. Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
    Science. 1992 Nov 13;258(5085):1112-8 PMID: 1279806
  26. Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication.
    Science. 1993 Jan 8;259(5092):234-8 PMID: 8421784
  27. A model system for nonhomologous recombination between retroviral and cellular RNA.
    J Virol. 1993 Jul;67(7):3845-53 PMID: 7685401
  28. Retrovirus variation and reverse transcription: abnormal strand transfers result in retrovirus genetic variation.
    Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):6900-3 PMID: 7688465
  29. Mutation of an RSV intronic element abolishes both U11/U12 snRNP binding and negative regulation of splicing.
    Genes Dev. 1993 Oct;7(10):1926-36 PMID: 8405999
  30. One retroviral RNA is sufficient for synthesis of viral DNA.
    J Virol. 1994 Jan;68(1):207-16 PMID: 8254730
  31. RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency.
    Cell. 1993 Dec 31;75(7):1361-70 PMID: 8269514
  32. Loss of conserved cysteine residues in the attachment (G) glycoprotein of two human respiratory syncytial virus escape mutants that contain multiple A-G substitutions (hypermutations).
    Virology. 1994 Feb;198(2):653-62 PMID: 7507282
  33. Fidelity of in vitro DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
    Biochemistry. 1994 Apr 5;33(13):3890-5 PMID: 7511410
  34. G-->A hypermutation of the human immunodeficiency virus type 1 genome: evidence for dCTP pool imbalance during reverse transcription.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3092-6 PMID: 7512722
  35. Nucleotide composition as a driving force in the evolution of retroviruses.
    J Mol Evol. 1994 May;38(5):506-32 PMID: 8028030
  36. An avian oncovirus mutant (SE 21Q1b) deficient in genomic RNA: biological and biochemical characterization.
    Cell. 1978 Dec;15(4):1371-81 PMID: 83199
  37. cis-Acting RNA packaging locus in the 115-nucleotide direct repeat of Rous sarcoma virus.
    J Virol. 1983 Dec;48(3):667-75 PMID: 6313966
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.9.5878-5882.1995
Published
1995-09-00
Pages
5878-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189466
Subset
IM
Grants
NCI NIH HHS · CA18282 · United States
NCI NIH HHS · P30CA15704 · United States
NCRR NIH HHS · RR00079 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.9.5878-5882.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2