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PMID: 7565798 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A class of activation domains interacts directly with TFIIA and stimulates TFIIA-TFIID-promoter complex assembly.

Molecular and cellular biology ·Vol. 15 ·No. 11 ·1995-11-00 ·Pages 6465-73

Kobayashi N, Boyer TG, Berk AJ

Abstract

TFIIA is a general transcription factor that interacts with the TFIID-promoter complex required for transcription initiation by RNA polymerase II. Two lines of evidence suggest that TFIIA is directly involved in the mechanism by which some activators stimulate transcription. First, binding of TFIIA to a TFIID-promoter complex is a rate-limiting step that is enhanced by transcriptional activators GAL4-AH and Zta. Second, recombinant TFIIA greatly enhances activator-dependent transcription. In this study, we found that the activation domains of Zta and VP16 bind directly to TFIIA. Both Zta and VP16 stimulated rapid assembly of a stable TFIID-TFIIA complex on promoter DNA. Analysis of deletion derivatives of the VP16 activation domain indicated that the ability to bind to TFIIA correlates with the ability to enhance TFIID-TFIIA-promoter ternary complex assembly. Thus, we propose that a class of activators stimulate transcription initiation through direct interactions with both TFIIA and TFIID, which stimulate the assembly of an activated TFIIA-TFIID-promoter complex.

MeSH Terms
Amino Acid Sequence DNA Footprinting DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Herpes Simplex Virus Protein Vmw65/metabolism Kinetics Macromolecular Substances Molecular Sequence Data Promoter Regions, Genetic Protein Binding RNA Polymerase II/metabolism Trans-Activators/metabolism Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors/metabolism Transcriptional Activation Viral Proteins
Chemicals
BZLF1 protein, Herpesvirus 4, Human DNA-Binding Proteins Fungal Proteins Gal-VP16 Herpes Simplex Virus Protein Vmw65 Macromolecular Substances Trans-Activators Transcription Factor TFIIA Transcription Factor TFIID Transcription Factors Viral Proteins RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kobayashi N
Molecular Biology Institute, University of California, Los Angeles 90024-1570, USA.
Boyer T G
Berk A J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.11.6465
Published
1995-11-00
Pages
6465-73
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230897
Subset
IM
Grants
NCI NIH HHS · CA25235 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.11.6465 PMC Full Text View journal details
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